Wolfgang Küpker, M. D. , Ph. D. , Peter N Schlegel, M. D

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Presentation transcript:

Use of frozen-thawed testicular sperm for intracytoplasmic sperm injection Wolfgang Küpker, M.D., Ph.D., Peter N Schlegel, M.D., Safaa Al-Hasani, Ph.D., Paolo Fornara, M.D., Ph.D., Rainer Johannisson, Ph.D., Jörg Sandmann, M.D., Thilo Schill, M.D., Monika Bals-Pratsch, M.D., Michael Ludwig, M.D., Klaus Diedrich, M.D., Ph.D. Fertility and Sterility Volume 73, Issue 3, Pages 453-458 (March 2000) DOI: 10.1016/S0015-0282(99)00564-6

Figure 1 Open testicular biopsy (two biopsy sites). Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility 2000 73, 453-458DOI: (10.1016/S0015-0282(99)00564-6)

Figure 2 Extracted testicular tissue before freezing. Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility 2000 73, 453-458DOI: (10.1016/S0015-0282(99)00564-6)

Figure 3 Sertoli cell–only pattern with focal spermatogenesis. Toluidine blue/pyronine stain. Original magnification, ×1,000). Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility 2000 73, 453-458DOI: (10.1016/S0015-0282(99)00564-6)

Figure 4 Protocol for freezing and thawing of testicular tissue. Küpker. Frozen-thawed sperm. Fertil Steril 2000. Fertility and Sterility 2000 73, 453-458DOI: (10.1016/S0015-0282(99)00564-6)