Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP,

Slides:

Advertisements

Similar presentations

Figure 7. Interaction between C9orf72 dipeptides and ribonucleotides in vivo. The concentration of RNA extracted from immunoprecipitated samples from NSC34.

Advertisements

Poly-GR and poly-PR interact with overlapping proteins in primary neurons and in HEK293 cells. Poly-GR and poly-PR interact with overlapping proteins in.

STAU1/2 and YBX1 recruit poly-GR/PR into large cytoplasmic granules.

Overexpression of RNase H1 decreases the induction of phosphorylated H2AX in post‐mitotic cells in response to topoisomerase 1 cleavage complexes. Overexpression.

Exposure to stress and SGs formation.

NPM1 drives poly-GR into the nucleolus of primary neurons.

Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients. Poly-GR and poly-PR co-aggregate with ribosomal proteins in C9orf72 patients.

Cytoplasmic poly-GR/PR inclusions resemble stress granules in vitro.

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Density of dendritic spines on L2/3 pyramidal neurons is greater in visual cortex of germline PirB−/− mice than in PirB+/+ mice at P30. Density of dendritic.

Poly-GR mainly co-localizes with nucleolar ribosomes.

Poly-GR and poly-PR interact with similar low-complexity proteins in neurons. Poly-GR and poly-PR interact with similar low-complexity proteins in neurons.

Figure 1 Reactivity of the patients' antibodies with rat brain and HEK cell-based assays Rat hippocampal dentate gyrus neuropils were stained with patient.

The β-tubulin C-terminal tail modulates the coordination of microtubule assembly in an isotype-dependent manner. The β-tubulin C-terminal tail modulates.

Poly-PR inhibits translation in neurons.

LATS2 promotes death of lumB cells.

Atp1a3 phosphomutants show alterations in subcellular localization.

Arc expression increases H4K12Ac levels.

Johanna Sigl-Glöckner, Michael Brecht Cell Reports

AG-221 can reduce intracellular 2HG levels and induce differentiation in primary human IDH2R140Q- or IDH2R172K-mutant AML patient samples treated ex vivo.

Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. Nucleolar poly-GR/PR alter nucleolar organization and inhibit translation. GFP,

Fig. 5. pKL cells abrogate the autoimmune response in vitro.

The absence of the centrosome changes global microtubule dynamics in a migrating cell. The absence of the centrosome changes global microtubule dynamics.

Axonal swelling and impairment of dendritic development in Purkinje cells from Pex14ΔC/ΔC BL/ICR mouse upon treatment with BDNF. Axonal swelling and impairment.

A. A. The two independent mutations identified in the structural gene of pqn-82 are shown above the gene. The genomic coordinates for the altered base.

Subcellular localization of UUKV, HRTV, and SFTSV NSs

An siRNA screen identifies BER pathway members as sensitizers to temozolomide (TMZ) in pediatric GBM. A and B, SJG2 and KNS42 cells were transfected with.

Modification of the βIII-tubulin C-terminal tail does not affect the incorporation of the protein into the microtubule network, the microtubule architecture,

V-SVZ cultures express transcription factors expected for region of origin. V-SVZ cultures express transcription factors expected for region of origin.

Establishment of monoclonal SMN2-GFP reporter line in HEK293.

Cellular transformation up-regulates proteins that help preserve telomere integrity. Cellular transformation up-regulates proteins that help preserve telomere.

Volume 2, Issue 3, Pages (March 2014)

The 3′F allele does not recapitulate the systemic hyperimmune phenotypes associated with mir-146a deficiency. The 3′F allele does not recapitulate the.

Indirect fluorescence assay of N. gruberi cells.

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. Reversal of MOR lysosomal targeting by silencing enhanced Rab7 expression. A:

MET activation increases the density of Syn-GFP/bassoon and Syn-GFP/PSD-95 clusters in neocortical neurons. MET activation increases the density of Syn-GFP/bassoon.

Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence. Ectopic expression of wt-DAO, but not the inactive mutant, promotes senescence.

MET/β-catenin complexes during synapse development.

Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.

Ventral mouse V-SVZ cells exhibit higher mTORC1 activity than dorsal cells and are responsive to rapamycin. Ventral mouse V-SVZ cells exhibit higher mTORC1.

Validation of GFP and BMP-2 expression in lentivirus-transduced neonatal human skin fibroblasts. Validation of GFP and BMP-2 expression in lentivirus-transduced.

TOR, but not OSP, blocks STx2B transport and alters endosomal dynamics

Socs1 KD tumors exhibit a more T cell–inflamed phenotype and increased CD8+ T cell activation. Socs1 KD tumors exhibit a more T cell–inflamed phenotype.

HGF recruits additional synapsin 1 to MET receptor complex.

Increased network ensembles and altered Ca2+ dynamics in Panx1 KO cortical neurons. Increased network ensembles and altered Ca2+ dynamics in Panx1 KO cortical.

KIF13A overexpression favors NP distribution to the cell edge and requires KIF13A binding to microtubules. KIF13A overexpressionfavorsNP distribution to.

Altered luminal pH of the Golgi apparatus in GPHR deficient primary cultured neurons. Altered luminal pH of the Golgi apparatus in GPHR deficient primary.

Fig. 1. Rnd2 and Rnd3 induce stress fibres whereas Rnd1 reduces stress fibres in endothelial cells.(A) Rnd mRNAs are expressed in HUVECs. Total RNA was.

FUT1 and STAM are required for the retrograde transport of STx2B to the Golgi. FUT1 and STAM are required for the retrograde transport of STx2B to the.

Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons. Metformin (Met) reduces membrane-associated TRPA1 levels in rat DRG neurons.

Sensory neurons grow and downregulate UCHL1 expression levels during postnatal maturation. Sensory neurons grow and downregulate UCHL1 expression levels.

Removal of both B-1–mediated anti-PC natural IgM and B-2–mediated anti-PEG IgM responses abrogates ABC in mice. Removal of both B-1–mediated anti-PC natural.

Fig. 7. Analysis of dFMRP kinetics in dFMRP granules by FRAP

Lack of LC3–GFP clustering in neurons expressing mutant CHMP2B.

LILRA3 increased density of synaptic contacts in mouse cortical neurons cultured for 21 days. LILRA3 increased density of synaptic contacts in mouse cortical.

Co-expression of VSVG–Cdc42-CA, but not VSVG–Cdc42-DN with eGFP–gephyrin (green) and Myc–CB2ΔPH (blue) rescues postsynaptic gephyrin clustering, as shown.

ADCY5 is expressed in isolated human islets and affected by T2D risk alleles. ADCY5 is expressed in isolated human islets and affected by T2D risk alleles.

Fig. 2 ODN enhances EV transfer between cells expressing TLR9.

S-Affs colocalize with SUMO in mammalian cells.

Activity regulates eEF2K-dependent spine morphogenesis.

ABC effect in WT or T cell– or B cell–deficient mice.

SSC LTP in narrow-field vertical neurons is induced by SO stimulation at 20 Hz for 20 s. sSC LTP in narrow-field vertical neurons is induced by SO stimulation.

Fig. 3 C9ORF72 interacts with SMCR8 in a DENN domain–dependent manner.

Fig. 1. CCR5-TALEN and CCR5-megaTAL activity in T cells and comparison of NHEJ and HDR events in a TLR reporter line. CCR5-TALEN and CCR5-megaTAL activity.

Quantification of TUNEL staining and OS length among the rab11a constructs. Quantification of TUNEL staining and OS length among the rab11a constructs.

Cellular localization of Panx1 and altered membrane morphology in stable Panx1-transfected C6 glioma cells. Cellular localization of Panx1 and altered.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

EGF induces HPSE nucleolar localization in human BMBC cells.

Presentation transcript:

Characterization of DPR proteins in primary rat neurons and HEK293 cells. Characterization of DPR proteins in primary rat neurons and HEK293 cells. GFP, GFP-(GR)149, or (PR)175-GFP were expressed in primary rat neurons and HEK293 cells. (A) Images show GFP fluorescence of a single confocal plane in cells stained with DAPI to visualize nuclei and anti-fibrillarin (red) to visualize nucleoli. Scale bar denotes 20 μm. (B) Quantitative analysis of poly-GR/PR localization to fibrillarin-positive nucleoli. Average co-localization from n = 17 for GFP-(GR)149, n = 13 for (PR)175-GFP images in neurons and n = 6 for GFP-(GR)149, n = 6 for (PR)175-GFP images in HEK cells from two independent experiments, mean ± SEM is shown. (C) LDH release assay detects significant toxic effect on (PR)175-GFP but not GFP-(GR)149 expression compared with GFP control in primary rat neurons (DIV7 + 14) (18 replicates from three independent experiments; box plot is shown with 25th percentile, median, and 75th percentile; and whiskers representing minimum and maximum, exact P-values: GFP versus GFP-GR149, P = 0.9583 and GFP versus PR175-GFP; P = 0.0001 in unpaired one-way ANOVA with Dunnett’s posttest). (D) Cell viability of HEK293 cells transfected with GFP, GFP-(GR)149, or (PR)175-GFP measured by XTT assay 3 days after transfection. GFP-(GR)149 and (PR)175-GFP showed significantly reduced viability compared with GFP control (12 replicates from two independent experiments; box plot is shown, exact P-values: GFP versus GFP-GR149, P = 0.0001 and GFP versus PR175-GFP, P = 0.0001 in unpaired one-way ANOVA with Dunnett’s posttest). Hannelore Hartmann et al. LSA 2018;1:e201800070 © 2018 Hartmann et al.