High resolution melting of Short Tandem Repeats amplicons

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High resolution melting of Short Tandem Repeats amplicons Dominika Kadlčíková, Karel Fusek, Jiří Drábek Forensic Science International: Genetics Supplement Series Volume 6, Pages e335-e336 (December 2017) DOI: 10.1016/j.fsigss.2017.09.120 Copyright © 2017 Elsevier B.V. Terms and Conditions

Fig. 1 Comparison of HRM curves of STR amplicons at different conditions. Thermocyclers: 1 – LC480 II, 2 – CFX96; PCR cocktails: 1 – ThermoScientific, 2 – Promega; Dilution series on 3 DNAs: 1 – vWA, first DNA, 2 – vWA, second DNA, 3 – vWA, third DNA, 4 – D18S51, first DNA, 5 – D18S51, second DNA, 6 – D18S51, third DNA; DNA cleaning by precipitation: 1 – vWA DNA extracted using QiaGen kit, 2 – vWA extracted DNA cleaned by ethanol precipitation and resuspending, 3 – D18S51 DNA extracted using QiaGen kit, 4 – D18S51 extracted DNA cleaned by ethanol precipitation and resuspending. Forensic Science International: Genetics Supplement Series 2017 6, e335-e336DOI: (10.1016/j.fsigss.2017.09.120) Copyright © 2017 Elsevier B.V. Terms and Conditions