Evidence of a role for B cell–activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps Atsushi Kato, PhD,

Slides:

Advertisements

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions Kathleen R. Bartemes, BA, Kate.

Advertisements

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.

Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3 Feng Lan, MD, Nan Zhang,

Russell S. Traister, MD, PhD, Crystal E. Uvalle, BS, Gregory A

Forkhead box protein 3 in human nasal polyp regulatory T cells is regulated by the protein suppressor of cytokine signaling 3 Feng Lan, MD, Nan Zhang,

Proton pump inhibitors decrease eotaxin-3/CCL26 expression in patients with chronic rhinosinusitis with nasal polyps: Possible role of the nongastric.

The antimicrobial protein short palate, lung, and nasal epithelium clone 1 (SPLUNC1) is differentially modulated in eosinophilic and noneosinophilic chronic.

Heterogeneous inflammatory patterns in chronic rhinosinusitis without nasal polyps in Chicago, Illinois Bruce K. Tan, MD, MS, Aiko I. Klingler, PhD,

Epigenetic regulation of established human type 1 versus type 2 cytokine responses Ruey-Chyi Su, PhD, Allan B. Becker, MD, Anita L. Kozyrskyj, PhD, Kent.

Evidence for intranasal antinuclear autoantibodies in patients with chronic rhinosinusitis with nasal polyps Bruce K. Tan, MD, Quan-Zhen Li, PhD, Lydia.

IL-25 as a novel therapeutic target in nasal polyps of patients with chronic rhinosinusitis Hyun-Woo Shin, MD, PhD, Dong-Kyu Kim, MD, Min-Hyun Park, MD,

Anju T. Peters, MD, Atsushi Kato, PhD, Ning Zhang, PhD, David B

IL-32 is expressed by human primary keratinocytes and modulates keratinocyte apoptosis in atopic dermatitis Norbert Meyer, MD, Maya Zimmermann, PhD,

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells Shigeru Okumura, DDS, PhD, Hironori Sagara,

Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3 Se Jin Park,

Expression of 11β-hydroxysteroid dehydrogenase 1 and 2 in patients with chronic rhinosinusitis and their possible contribution to local glucocorticoid.

Selective and direct activation of human neutrophils but not eosinophils by Toll-like receptor 8 Markus Janke, PhD, Jens Poth, Vera Wimmenauer, Thomas.

Dawn C. Newcomb, PhD, Madison G

Oncostatin M promotes mucosal epithelial barrier dysfunction, and its expression is increased in patients with eosinophilic mucosal disease Kathryn L.

T-cell regulation in chronic paranasal sinus disease

Role of TWIK-related potassium channel-1 in chronic rhinosinusitis

Evidence for altered levels of IgD in the nasal airway mucosa of patients with chronic rhinosinusitis Jin-Young Min, MD, PhD, Jayakar V. Nayak, MD, PhD,

Neutrophils are a major source of the epithelial barrier disrupting cytokine oncostatin M in patients with mucosal airways disease Kathryn L. Pothoven,

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

Role of p63/p73 in epithelial remodeling and their response to steroid treatment in nasal polyposis Chun Wei Li, PhD, Li Shi, MD, Ke Ke Zhang, MD, Tian.

Reply The Journal of Allergy and Clinical Immunology: In Practice

Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps Sarah Peterson, MD, Julie A. Poposki, MS, Deepti.

Susan C. Foley, MD, David Préfontaine, MSc, Qutayba Hamid, MD, PhD

Griet A. Van Roey, PhD, Christopher C

Kathleen R. Bartemes, BA, Gail M. Kephart, BS, Stephanie J

Corticosteroid insensitivity of chemokine expression in airway smooth muscle of patients with severe asthma Po-Jui Chang, MD, Pankaj K. Bhavsar, PhD,

Dexamethasone regulation of thymic stromal lymphopoietin receptor expression on mast cells and their precursors Zoulfia Allakhverdi, PhD, Michael R.

RAGE processing in chronic airway conditions: Involvement of Staphylococcus aureus and ECP Koen Van Crombruggen, PhD, Gabriele Holtappels, Natalie De.

Cross-talk between human mast cells and epithelial cells by IgE-mediated periostin production in eosinophilic nasal polyps Dae Woo Kim, MD, PhD, Marianna.

Corticosteroid-resistant asthma is associated with classical antimicrobial activation of airway macrophages Elena Goleva, PhD, Pia J. Hauk, MD, Clifton.

Fibronectin is a TH1-specific molecule in human subjects

Cigarette smoke combined with Toll-like receptor 3 signaling triggers exaggerated epithelial regulated upon activation, normal T-cell expressed and secreted/CCL5.

David D. Tieu, MD, Anju T. Peters, MD, Roderick T

TGF-β signaling and collagen deposition in chronic rhinosinusitis

Zoulfia Allakhverdi, PhD, Michael R. Comeau, BSc, Dirk E

Microparticles in nasal lavage fluids in chronic rhinosinusitis: Potential biomarkers for diagnosis of aspirin-exacerbated respiratory disease Toru Takahashi,

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

Increased expression of the epithelial anion transporter pendrin/SLC26A4 in nasal polyps of patients with chronic rhinosinusitis Sudarshan Seshadri,

Downregulation of a disintegrin and metalloproteinase 33 by IFN-γ in human airway smooth muscle cells Isao Ito, MD, PhD, Johanne D. Laporte, PhD, Pierre.

Prostaglandin E2 suppresses staphylococcal enterotoxin–induced eosinophilia- associated cellular responses dominantly through an E-prostanoid 2–mediated.

Airway epithelial cells activate TH2 cytokine production in mast cells through IL-1 and thymic stromal lymphopoietin Deepti R. Nagarkar, PhD, Julie A.

Sudarshan Seshadri, PhD, Mariel Rosati, BS, David C

Chronic rhinosinusitis with nasal polyps is characterized by B-cell inflammation and EBV-induced protein 2 expression Kathryn E. Hulse, PhD, James E.

Fibronectin is a TH1-specific molecule in human subjects

Increased noneosinophilic nasal polyps in chronic rhinosinusitis in US second- generation Asians suggest genetic regulation of eosinophilia Mahboobeh.

Staphylococcal exotoxins are strong inducers of IL-22: A potential role in atopic dermatitis Margarete Niebuhr, MD, Helena Scharonow, MS, Merle Gathmann,

Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps Tetsuji Takabayashi, MD, Atsushi Kato, PhD,

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

IL-25 as a novel therapeutic target in nasal polyps of patients with chronic rhinosinusitis Hyun-Woo Shin, MD, PhD, Dong-Kyu Kim, MD, Min-Hyun Park, MD,

Lipoteichoic acid downregulates FcεRI expression on human mast cells through Toll-like receptor 2 Mino Yoshioka, MSc, Nobuyuki Fukuishi, PhD, Sayuri.

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Matthew J. Loza, PhD, Susan Foster, PhD, Stephen P

TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis Shin Morizane, MD, PhD, Kenshi Yamasaki, MD, PhD, Ai Kajita,

Correlation between CCL26 production by human bronchial epithelial cells and airway eosinophils: Involvement in patients with severe eosinophilic asthma

Mucin 1 downregulation associates with corticosteroid resistance in chronic rhinosinusitis with nasal polyps Javier Milara, PhD, PharmD, Teresa Peiró,

Biology of nasal polyposis

Human rhinovirus infection enhances airway epithelial cell production of growth factors involved in airway remodeling Richard Leigh, MBChB, PhD, Wale.

IL-10, TGF-β, and glucocorticoid prevent the production of type 2 cytokines in human group 2 innate lymphoid cells Noriko Ogasawara, MD, PhD, Julie A.

Age-related differences in the pathogenesis of chronic rhinosinusitis

Inflammation-mediated upregulation of centrosomal protein 110, a negative modulator of ciliogenesis, in patients with chronic rhinosinusitis Yinyan Lai,

Cigarette smoke exposure is associated with vitamin D3 deficiencies in patients with chronic rhinosinusitis Jennifer K. Mulligan, PhD, Whitney Nagel,

Karl J. Staples, PhD, Timothy S. C. Hinks, MB BS, Jon A

CCL17/thymus and activation-regulated chemokine induces calcitonin gene–related peptide in human airway epithelial cells through CCR4 Kandace Bonner,

Presentation transcript:

Evidence of a role for B cell–activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps Atsushi Kato, PhD, Anju Peters, MD, Lydia Suh, BSc, Roderick Carter, BSc, Kathleen E. Harris, BSc, Rakesh Chandra, MD, David Conley, MD, Leslie C. Grammer, MD, Robert Kern, MD, Robert P. Schleimer, PhD Journal of Allergy and Clinical Immunology Volume 121, Issue 6, Pages 1385-1392.e2 (June 2008) DOI: 10.1016/j.jaci.2008.03.002 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Detection of BAFF in patients with CRSwNP. A, Total RNA was extracted from IT and nasal polyps (Polyp), and expression of BAFF was analyzed by using real-time PCR. The concentration of BAFF in tissue homogenates of IT and polyp (B) and nasal lavage (C) was measured by using ELISA. BAFF concentration was normalized to the concentration of total protein. Journal of Allergy and Clinical Immunology 2008 121, 1385-1392.e2DOI: (10.1016/j.jaci.2008.03.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Immunohistochemistry of BAFF was performed by using antihuman BAFF mAb (clone: Buffy 2). A, Negative control antibody staining in uncinate tissue (UT) from a patient with CRSsNP. B-F, Representative immunostaining for BAFF in UT from a control subject (B), a patient with CRSsNP (C), and a patient with CRSwNP (D) and in nasal polyp tissue (E and F). Arrow, Eosinophil-like cells. Magnification ×200 (A-E); ×400 (F). Journal of Allergy and Clinical Immunology 2008 121, 1385-1392.e2DOI: (10.1016/j.jaci.2008.03.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 B-cell accumulation in nasal polyps. A, Total RNA was extracted from IT and nasal polyps (Polyp), and expression of CD20 was analyzed by using real-time PCR. B, Relationship of expression of BAFF and the B-cell marker CD20 or the BAFF receptor TACI in the nasal tissue was evaluated by using real-time PCR. Correlations were assessed by using the Spearman rank correlation. Journal of Allergy and Clinical Immunology 2008 121, 1385-1392.e2DOI: (10.1016/j.jaci.2008.03.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Elevated expression of IgA in nasal polyps. Measurement of IgA in tissue homogenates of IT from control subjects, from patients with CRSsNP and CRSwNP, and in nasal polyps was measured by using ELISA. IgA concentration was normalized to the concentration of total protein. Journal of Allergy and Clinical Immunology 2008 121, 1385-1392.e2DOI: (10.1016/j.jaci.2008.03.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Expression of BAFF and APRIL in nasal epithelial cells Expression of BAFF and APRIL in nasal epithelial cells. A, PNECs were incubated for 6 hours with 10 μg/mL peptidoglycan (PGN), 25 μg/mL dsRNA, 1 μg/mL LPS, 100 ng/mL TNF, 100 ng/mL IL-4, 1000 U/mL IFN-β, 10 ng/mL IFN-γ, 100 ng/mL IFN-λ1, and 100 ng/mL oncostatin M (OSM) as indicated, and then mRNA was extracted and analyzed for BAFF and APRIL using real-time PCR. The mRNA expression levels were normalized to the median expression of the housekeeping gene, ACTB. B, PNECs were incubated with dsRNA, 1000 U/mL IFN-β, 10 ng/mL IFN-γ, 100 ng/mL IFN-λ1, or vehicle control (medium) for 6 to 72 hours, and then expression of mRNA for BAFF was analyzed by real-time PCR. C, Detection of BAFF protein by ELISA in the culture supernatant of PNECs stimulated with dsRNA and cytokines for 24 or 72 hours. Results shown are means ± SEMs of 4 to 7 independent experiments. ND, Not detectable. ∗P < .05. Journal of Allergy and Clinical Immunology 2008 121, 1385-1392.e2DOI: (10.1016/j.jaci.2008.03.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions