by Bart L. Haagmans, Judith M. A. van den Brand, V

Slides:

Advertisements

Similar presentations

Deletion of Irf3 and Irf7 Genes in Mice Results in Altered Interferon Pathway Activation and Granulocyte-Dominated Inflammatory.

Advertisements

Reliable typing of MERS-CoV variants with a small genome fragment

Molecular Therapy - Methods & Clinical Development

Long-term detection of seasonal influenza RNA in faeces and intestine

Neonatal Oxygen Increases Sensitivity to Influenza A Virus Infection in Adult Mice by Suppressing Epithelial Expression of Ear1 Michael A. O'Reilly,

Representative hematoxylin-eosin stained (HE) sections demonstrating immune checkpoint inhibition-related colitis representative HE sections demonstrating.

Volume 49, Issue 1, Pages (January 2006)

Airborne Transmission of Influenza A/H5N1 Virus Between Ferrets

Analysis of a Successful Immune Response against Hepatitis C Virus

Fig. 6. AZD6738 induces DNA damage and apoptosis and exhibits antitumor efficacy in xenograft models of high-risk medulloblastoma and neuroblastoma. AZD6738.

Volume 44, Issue 6, Pages (June 2016)

Volume 16, Issue 5, Pages (August 2016)

In Vivo Mechanisms of Vaccine-Induced Protection against HPV Infection

Long-term detection of seasonal influenza RNA in faeces and intestine

Targeting lentiviral vector expression to hepatocytes limits transgene-specific immune response and establishes long-term expression of human antihemophilic.

by Angela L. Rasmussen, Atsushi Okumura, Martin T

The effect of bisphenol A (BPA) on colon and liver inflammation.

In vivo function of MeTro sealants using rat incision model of lungs

Volume 124, Issue 5, Pages (May 2003)

Respiratory syncytial virus nephropathy in rats

Fig. 2. Engraftment of CART-EGFRvIII and cytokine modulation in the peripheral blood. Engraftment of CART-EGFRvIII and cytokine modulation in the peripheral.

Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating lymphocytes. Geographic distribution in situ of MSI and MSS colorectal cancer–infiltrating.

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Fig. 8. mRIPO elicits neutrophil influx followed by DC and T cell infiltration into tumors. mRIPO elicits neutrophil influx followed by DC and T cell infiltration.

Fig. 4. Characterization of human liver seed graft morphology.

Immunofluorescence staining of virus-infected human placental explants

by Michael J. Mina, C. Jessica E. Metcalf, Rik L. de Swart, A. D. M. E

Volume 24, Issue 5, Pages (May 2016)

Volume 26, Issue 2, Pages (February 2018)

Expression of THBS2 in human PanIN tissue and PDAC tumor tissue

Type 1 immunity drives metabolic disease but protects against NAFLD

Volume 4, Issue 3, Pages (September 2008)

M. Svensson, H. Irjala, C. Svanborg, G. Godaly Kidney International

Volume 5, Issue 1, Pages (January 2009)

Endogenous polyclonal anti–IL-1 antibody responses potentiate IL-1 activity during pathogenic inflammation Gunther Spohn, PhD, Natalia Arenas-Ramirez,

Meta-Analysis of Hepatitis C Virus Vaccine Efficacy in Chimpanzees Indicates an Importance for Structural Proteins Harel Dahari, Stephen M. Feinstone,

Fig. 7. Inhibitory mechanisms of C12G6.

Stanford L Peng, Andrea J Gerth, Ann M Ranger, Laurie H Glimcher

Elysse C. Filipe et al. BTS 2018;3:38-53

Rapid development of a DNA vaccine for Zika virus

Volume 28, Issue 6, Pages (June 2008)

Sarita Sehra, PhD, Weiguo Yao, PhD, Evelyn T. Nguyen, MS, Nicole L

Volume 8, Issue 3, Pages (September 2003)

Volume 9, Issue 6, Pages (June 2004)

Volume 10, Issue 1, Pages (January 1999)

Human Dendritic Cells as Targets of Dengue Virus Infection

LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.

Fig. 3 Hepatic HBV envelope RNA and protein expression levels in HBVRplRag−/− mice 7 days (1 week) and 8 to 24 weeks after adoptive transfer with WT or.

Volume 16, Issue 5, Pages (August 2016)

Volume 22, Issue 1, Pages (January 2014)

Fig. 2 Adoptive transfer of adult Ox40−/− splenocytes into adult HBVEnvRag−/− mice alters hepatic inflammation, HBsAg clearance, HBsAb seroconversion,

by Asuka Hirai-Yuki, Lucinda Hensley, David R

Fig. 5. Prophylactic and therapeutic efficacy of C12G6 in ferrets.

Fig. 3 CSF1 is expressed in human melanoma.

Isolation and Characterization of Viruses Related to the SARS Coronavirus from Animals in Southern China by Y. Guan, B. J. Zheng, Y. Q. He, X. L. Liu,

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

Fig. 5 Treatment with an OX40 agonist antibody of 3-week-old HBVtgRag−/− mice or mice with chronic HBV disease results in an altered immune response to.

Fig. 4. CIZ1 reduces the impact of injury to the heart.

Autoimmune Intestinal Pathology Induced by hsp60-Specific CD8 T Cells

Anti-IgE efficacy in murine asthma models is dependent on the method of allergen sensitization Daniel B. Tumas, DVM, PhDa, Betty Chan, BSb, Winifred.

Alex KleinJan, PhD, Monique Willart, BSc, Leonie S

Antibody titers against surface antigens of B

Volume 21, Issue 3, Pages (March 2017)

GC reaction is impaired in NOTCH2 knock-in mice.

Volume 16, Issue 10, Pages (October 2008)

LAIV and WT influenza virus infection similarly enhance 19F pneumococcal carriage density and duration of colonization. LAIV and WT influenza virus infection.

Antibody titers against surface antigens of M

Volume 22, Issue 1, Pages (January 2014)

Lkt antibody titers of vaccinated and control groups of BHS

Reduced pathological damage in lungs and spleens of guinea pigs infected with H37Rv upon AZD0530 treatment. Reduced pathological damage in lungs and spleens.

Presentation transcript:

An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels by Bart L. Haagmans, Judith M. A. van den Brand, V. Stalin Raj, Asisa Volz, Peter Wohlsein, Saskia L. Smits, Debby Schipper, Theo M. Bestebroer, Nisreen Okba, Robert Fux, Albert Bensaid, David Solanes Foz, Thijs Kuiken, Wolfgang Baumgärtner, Joaquim Segalés, Gerd Sutter, and Albert D. M. E. Osterhaus Science Volume 351(6268):77-81 January 1, 2016 Published by AAAS

Fig. 1 Virus-neutralizing antibody responses to MERS-CoV, MVA, and camelpox virus in vaccinated dromedary camels. Virus-neutralizing antibody responses to MERS-CoV, MVA, and camelpox virus in vaccinated dromedary camels. (A to D) Individual virus neutralization titers (VNT) from dromedary camels vaccinated with PBS, MVA-wt, or MVA-S against MERS-CoV [(A) and (B)], MVA (C), and camelpox virus (D), as determined from sera [(A), (C), and (D)] and nasal swabs (B). Here, VNT is expressed as the ratio denominator only [i.e., 32 on the y axis of (A) represents 1:32]. Different symbols indicate time points after immunization sera were analyzed: week 0 (black circles), week 4 (blue triangles), and week 7 (red squares). Dashed lines depict the detection limit of the assays Bart L. Haagmans et al. Science 2016;351:77-81 Published by AAAS

Fig. 2 Clinical signs and MERS-CoV excretion in nasal swabs of dromedary camels vaccinated with MVA-S vaccine. Clinical signs and MERS-CoV excretion in nasal swabs of dromedary camels vaccinated with MVA-S vaccine. (A and B) Two MVA-S–vaccinated (A) and two control-vaccinated dromedary camels (B) were analyzed for the presence of mucus excretion 8 to 10 days after MERS-CoV challenge. (C and D) Detection of infectious MERS-CoV (C) and MERS-CoV RNA (D) at different time points after challenge in nasal swabs of dromedary camels vaccinated with MVA-S (white bars) or MVA-wt or PBS (black bars). Dashed lines depict the detection limit of the assays. Error bars represent mean values ± SEM; *P < 0.05; n = 4 animals per group. GE, genome equivalents. Bart L. Haagmans et al. Science 2016;351:77-81 Published by AAAS

Fig. 3 Detection of MERS-CoV in tissues of vaccinated dromedary camels. Detection of MERS-CoV in tissues of vaccinated dromedary camels. (A and B) Levels of MERS-CoV viral RNA (A) and infectious virus (B) were determined in tissue homogenates from MVA-S–vaccinated (green and black bars) or control-vaccinated (red and blue bars) camels 4 days after challenge. Bart L. Haagmans et al. Science 2016;351:77-81 Published by AAAS

Fig. 4 Histopathology and expression of viral antigen and viral RNA in the nasal respiratory epithelium of MVA-S–vaccinated and control-vaccinated dromedaries 4 days after challenge with MERS-CoV. Histopathology and expression of viral antigen and viral RNA in the nasal respiratory epithelium of MVA-S–vaccinated and control-vaccinated dromedaries 4 days after challenge with MERS-CoV. (A to C) Detection of MERS-CoV viral RNA by ISH in the noses of MVA-S–vaccinated (A) or control-vaccinated (B) dromedary camels. Nasal respiratory tissue of a representative MVA-S–vaccinated dromedary exhibited no prominent lesions (C), as revealed by staining with hematoxylin and eosin (HE). IHC and ISH results revealed a few viral antigen–positive cells and the presence of viral RNA, respectively. Nasal respiratory tissue of a control (Ctrl)–vaccinated dromedary exhibited multifocal necrosis of epithelial cells and infiltration of neutrophils, lymphocytes, and a few macrophages in the epithelium and lamina propria, with viral antigens and viral RNA present in abundance at the same location (C). Bart L. Haagmans et al. Science 2016;351:77-81 Published by AAAS