Review of Norwich Microarray Images and QC Why do we look at this image? -Good for seeing visual defects -Examining Borders, Chip ID, Controls Why do we look at the QC report? Check 3’ to 5’ ratios of housekeeping genes -Scaling factor -Spike in control signal So lets look at the images and see what happened… team by team
T1 GOOD !!
T2 GOOD !!
T3 GOOD !!
T4 GOOD !!
T5 GOOD !!
T6 GOOD !!
T7 GOOD !!
T8 No Good-Failed Rxn Just kidding GOOD !!
QC Report From Genechip Actin Housekeeping Control 3’-5’ Ratio TATA BP