A.G.G. Lødeng, C. Ahlén, H. Lysvand, L.H. Mandal, O.J. Iversen

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Presentation transcript:

Development and evaluation of a new PCR assay for detection of Pseudomonas aeruginosa D genotype A.G.G. Lødeng, C. Ahlén, H. Lysvand, L.H. Mandal, O.J. Iversen Clinical Microbiology and Infection Volume 12, Issue 8, Pages 761-768 (August 2006) DOI: 10.1111/j.1469-0691.2006.01482.x Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 1 Examples of RAPD-PCR analysis of Pseudomonas aeruginosa and other bacteria. Lanes: 2, 8, 14 and 19, 100-bp PCR molecular ruler; 1, Escherichia coli BL 21(DE3) control DNA; 20, E. coli C1a control DNA; 3, P. aeruginosa D1; 4, P. aeruginosa D2; 5, P. aeruginosa D10; 6, P. aeruginosa E1; 7, P. aeruginosa E4; 9, P. aeruginosa TP2-1; 10, P. aeruginosa TP2-2; 11, P. aeruginosa TP12-1; 12, P. aeruginosa TP12-2; 13, P. aeruginosa PAK; 15, Acinetobacter baumannii; 16, E. coli; 17, Klebsiella pneumoniae; 18, Proteus mirabilis. Clinical Microbiology and Infection 2006 12, 761-768DOI: (10.1111/j.1469-0691.2006.01482.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 2 Examples of PCR amplification of Pseudomonas aeruginosa and other bacteria with the two primer sets: (A) PCR products obtained with the primer set specific for P. aeruginosa genotype D (382-bp amplicon); (B) PCR products obtained with the primer set specific for P. aeruginosa (192-bp amplicon). Lanes: 1 and 20, 100-bp molecular ruler; 2, P. aeruginosa ATCC 27853; 3, P. aeruginosa CCUG 551 T; 4, P. aeruginosa PAO1; 5, P. aeruginosa D1; 6, P. aeruginosa TP2-1; 7, Pseudomonas mendocina CCUG 1781 T; 8, Pseudomonas stutzeri CCUG 11256 T; 9, Pseudomonas fluorescens CCUG 1253 T; 10, Pseudomonas alcaligenes CCUG 1425 A T; 11, Sphingomonas paucimobilis CCUG 6518 T; 12, Serratia marcescens CCUG 1647 T; 13, Stenotrophomonas maltophilia CCUG 5866 T; 14, Acinetobacter lwoffii CCUG 33984 T; 15, Comamonas aquatica CCUG 15845 T; 16, Escherichia coli; 17, Serratia fonticola CCUG 14186 T; 18, Proteus mirabilis; 19, negative control (no DNA added). Clinical Microbiology and Infection 2006 12, 761-768DOI: (10.1111/j.1469-0691.2006.01482.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions

Fig. 3 Amplicons generated from lysates of bacterial mixtures originating from freshwater samples from the diving environment following multiplex PCR with primer sets for Pseudomonas aeruginosa and P. aeruginosa genotype D. Lanes: 1, 100-bp molecular ruler; 2, P. aeruginosa D5 – extracted DNA (positive control); 3, sample 1; 4, sample 2; 5, sample 3; 6, sample 4; 7, sample 5; 8, sample 6; 9, sample 7; 10, negative control (no DNA added). Clinical Microbiology and Infection 2006 12, 761-768DOI: (10.1111/j.1469-0691.2006.01482.x) Copyright © 2006 European Society of Clinical Infectious Diseases Terms and Conditions