PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia  M. Dendis, R. Horváth, J. Michálek, F. Růžička, M. Grijalva, M. Bartoš, J. Benedík  Clinical Microbiology and Infection  Volume 9, Issue 12, Pages 1191-1202 (December 2003) DOI: 10.1111/j.1469-0691.2003.00719.x Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 (a) Physical map of the C. albicans rDNA region showing the positions of rRNA genes and interstitial NTS and ITS sequences (from [12]). (b) Expanded map of amplified ITS2 region showing the positions of primers, restriction sites and APLP polymorphism of (I) Candida albicans (II) Candida pelliculosa and (III) Mucor racemosus. Clinical Microbiology and Infection 2003 9, 1191-1202DOI: (10.1111/j.1469-0691.2003.00719.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 PCR–RFLP detection and species identification of fungal pathogens in blood of patients with febrile neutropenia. Ethidium bromide-stained agarose gels. (a) PCR detection of fungal ITS2 sequence in blood of patients using universal fungal primers UNF I and UNF2. 1, 4, 5, 7, 8 – positive patients (1-Aspergillus sp., 4-C albicans, 5-C.glabrata, 7-C tropicalis, 8-C krusei); 2, 3, 6 – negative patients; 9 – negative isolation control; 10 – positive control (101 copies of positive control plasmid); 11 – PCR negative control (distilled water added into the PCR reaction). 519 bp fragment – amplified internal standard. (b) RFLP patterns obtained after positive amplification of patient's products treated with HaeIII (a) and TaqI (b) endonucleases. 1 -Aspergillus sp., 2-C.glabrata, 3-C tropicalis, 4-G krusei, 5-G albicans. M- 20 bp molecular weight standard. Clinical Microbiology and Infection 2003 9, 1191-1202DOI: (10.1111/j.1469-0691.2003.00719.x) Copyright © 2003 European Society of Clinical Infectious Diseases Terms and Conditions