Diagnostic and Prognostic Value of Metastasis Inducer S100A4 Transcripts in Plasma of Colon, Rectal, and Gastric Cancer Patients Ulrike Stein, Susen.

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Diagnostic and Prognostic Value of Metastasis Inducer S100A4 Transcripts in Plasma of Colon, Rectal, and Gastric Cancer Patients Ulrike Stein, Susen Burock, Pia Herrmann, Ina Wendler, Markus Niederstrasser, Klaus-Dieter Wernecke, Peter M. Schlag The Journal of Molecular Diagnostics Volume 13, Issue 2, Pages 189-198 (March 2011) DOI: 10.1016/j.jmoldx.2010.10.002 Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 S100A4 transcripts in plasma of tumor-free volunteers. Box plot analysis, based on quantitative real-time RT-PCR (Tables 1–3). No different S100A4 transcript levels were found in two independently analyzed cohorts of tumor-free volunteers. The Journal of Molecular Diagnostics 2011 13, 189-198DOI: (10.1016/j.jmoldx.2010.10.002) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 S100A4 transcripts in plasma discriminate cancer patients from tumor-free volunteers. Box plot analysis, based on quantitative real-time RT-PCR (Tables 1–3). All patient cohorts with colorectal, colon, rectal, or gastric cancer expressed significantly higher S100A4 transcript levels than healthy volunteers. The Journal of Molecular Diagnostics 2011 13, 189-198DOI: (10.1016/j.jmoldx.2010.10.002) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 S100A4 transcripts in plasma of patients newly diagnosed with a primary tumor without or with synchronous metastasis, or with metachronous metastasis. Box plot analysis, based on quantitative real-time RT-PCR (Tables 1–3). All patients' subcohorts with colon (A), rectal (B), and gastric (C) cancer expressed significantly higher S100A4 transcript levels than healthy volunteers. Significantly different S100A4 levels were also found for colon (A) patients of stages I–III versus IV, and for colon cancer patients without and with metachronous metastasis (A). The Journal of Molecular Diagnostics 2011 13, 189-198DOI: (10.1016/j.jmoldx.2010.10.002) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 4 S100A4 transcripts in plasma of follow-up cancer patients without or with distant metastases. Box plot analysis, based on quantitative real-time RT-PCR (Tables 1–3). All follow-up patient subcohorts with nonmetastasized or metastasized colon (A), rectal (B), and gastric (C) cancer expressed significantly higher S100A4 transcript levels than healthy volunteers. Significantly different S100A4 levels were also found for rectal (B) follow-up patients without versus with metastasis; higher S100A4 levels were determined in patients with metastasized colon (A) and gastric (C) cancer compared with patients without metastasis. The Journal of Molecular Diagnostics 2011 13, 189-198DOI: (10.1016/j.jmoldx.2010.10.002) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 5 S100A4 transcripts in plasma of follow-up cancer patients and DFS. A: Kaplan–Meier analysis for DFS of all follow-up colorectal cancer patients (n = 288, Tables 1 and 2). Based on the median (0.387 S100A4 mRNA expression, % calibrator), all follow-up patients were subclassified into S100A4 transcript low (below median; n = 145) and high (above median; n = 143) expressors. DFS was significantly decreased in patients with high S100A4 transcript levels (P = 0.013). B: Kaplan-Meier analysis for DFS of all follow-up gastric cancer patients (n = 64, Table 3). Based on the median (0.39 S100A4 mRNA expression, % calibrator), all follow-up patients were subclassified into S100A4 transcript low (below median; n = 32) and high (above median; n = 32) expressors. The Journal of Molecular Diagnostics 2011 13, 189-198DOI: (10.1016/j.jmoldx.2010.10.002) Copyright © 2011 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions