Kinetic Analysis of Protein Crystal Nucleation in Gel Matrix

Slides:

Advertisements

Similar presentations

Creep Function of a Single Living Cell Nicolas Desprat, Alain Richert, Jacqueline Simeon, Atef Asnacios Biophysical Journal Volume 88, Issue 3, Pages

Advertisements

How Do Thermophilic Proteins and Proteomes Withstand High Temperature? Lucas Sawle, Kingshuk Ghosh Biophysical Journal Volume 101, Issue 1, Pages

Light-Scattering Studies of Protein Solutions: Role of Hydration in Weak Protein-Protein Interactions A. Paliwal, D. Asthagiri, D. Abras, A.M. Lenhoff,

Kinetic Properties of the Cardiac L-Type Ca2+ Channel and Its Role in Myocyte Electrophysiology: A Theoretical Investigation Gregory M. Faber, Jonathan.

Liposomes, Disks, and Spherical Micelles: Aggregate Structure in Mixtures of Gel Phase Phosphatidylcholines and Poly(Ethylene Glycol)-Phospholipids Markus.

Determination of the Interfacial Water Content in Protein-Protein Complexes from Free Energy Simulations Peter Monecke, Thorsten Borosch, Jürgen Brickmann,

The Dynamics and Mechanics of Endothelial Cell Spreading Cynthia A. Reinhart-King, Micah Dembo, Daniel A. Hammer Biophysical Journal Volume 89, Issue 1,

Coarse-Grained Molecular Simulation of Diffusion and Reaction Kinetics in a Crowded Virtual Cytoplasm Douglas Ridgway, Gordon Broderick, Ana Lopez-Campistrous,

High-Resolution Waveguide THz Spectroscopy of Biological Molecules N. Laman, S. Sree Harsha, D. Grischkowsky, Joseph S. Melinger Biophysical Journal Volume.

Structure of the Alamethicin Pore Reconstructed by X-Ray Diffraction Analysis Shuo Qian, Wangchen Wang, Lin Yang, Huey W. Huang Biophysical Journal Volume.

The Nuclear Pore Complex Mystery and Anomalous Diffusion in Reversible Gels Thomas Bickel, Robijn Bruinsma Biophysical Journal Volume 83, Issue 6, Pages.

A Hydrodynamic Model for Hindered Diffusion of Proteins and Micelles in Hydrogels Ronald J. Phillips Biophysical Journal Volume 79, Issue 6, Pages

Spatiotemporal Image Correlation Spectroscopy (STICS) Theory, Verification, and Application to Protein Velocity Mapping in Living CHO Cells Benedict Hebert,

Mesoscale Simulation of Blood Flow in Small Vessels Prosenjit Bagchi Biophysical Journal Volume 92, Issue 6, Pages (March 2007) DOI: /biophysj

Cell Traction Forces Direct Fibronectin Matrix Assembly Christopher A. Lemmon, Christopher S. Chen, Lewis H. Romer Biophysical Journal Volume 96, Issue.

Design of Superior Spider Silk: From Nanostructure to Mechanical Properties Ning Du, Xiang Yang Liu, Janaky Narayanan, Lian Li, Matthew Lek Min Lim, Daiqin.

Mechanical Properties of Spider Dragline Silk: Humidity, Hysteresis, and Relaxation T. Vehoff, A. Glišović, H. Schollmeyer, A. Zippelius, T. Salditt Biophysical.

Agarose-Dextran Gels as Synthetic Analogs of Glomerular Basement Membrane: Water Permeability Jeffrey A. White, William M. Deen Biophysical Journal Volume.

Phenol Red Interacts with the Protofibril-Like Oligomers of an Amyloidogenic Hexapeptide NFGAIL through Both Hydrophobic and Aromatic Contacts Chun Wu,

Near-Critical Behavior of Aminoacyl-tRNA Pools in E. coli at Rate-Limiting Supply of Amino Acids Johan Elf, Måns Ehrenberg Biophysical Journal Volume 88,

Different Effects of Trifluoroethanol and Glycerol on the Stability of Tropomyosin Helices and the Head-to-Tail Complex Fernando Corrêa, Chuck S. Farah.

Adsorption and function of recombinant Factor VIII at the air–water interface in the presence of Tween 80 Omkar Joshi, Liping Chu, Joseph McGuire, D.Q.

Mehdi Bagheri Hamaneh, Matthias Buck Biophysical Journal

Interaction of LL-37 with Model Membrane Systems of Different Complexity: Influence of the Lipid Matrix E. Sevcsik, G. Pabst, W. Richter, S. Danner,

A Dynamics Criterion to Determine Allostery

Electroosmosis in Transdermal Iontophoresis: Implications for Noninvasive and Calibration-Free Glucose Monitoring Anke Sieg, Richard H. Guy, M. Begoña.

Computer Simulation of Small Molecule Permeation across a Lipid Bilayer: Dependence on Bilayer Properties and Solute Volume, Size, and Cross-Sectional.

Urea-Induced Unfolding of the Immunity Protein Im9 Monitored by spFRET

Volume 109, Issue 11, Pages (December 2015)

Influence of Chain Length and Unsaturation on Sphingomyelin Bilayers

Volume 89, Issue 1, Pages (July 2005)

Behavior of Giant Vesicles with Anchored DNA Molecules

Avanish S. Parmar, Martin Muschol Biophysical Journal

Dynamics of Membrane Nanotubulation and DNA Self-Assembly

Henry van den Bedem, Ellen Kuhl Biophysical Journal

Volume 95, Issue 8, Pages L51-L53 (October 2008)

Gel or Die: Phase Separation as a Survival Strategy

Reversible Phosphorylation Subserves Robust Circadian Rhythms by Creating a Switch in Inactivating the Positive Element Zhang Cheng, Feng Liu, Xiao-Peng.

Volume 103, Issue 11, Pages (December 2012)

Volume 87, Issue 2, Pages (August 2004)

Volume 86, Issue 4, Pages (April 2004)

Avanish S. Parmar, Martin Muschol Biophysical Journal

Volume 101, Issue 7, Pages (October 2011)

Christopher Deufel, Michelle D. Wang Biophysical Journal

Membrane Thickening by the Antimicrobial Peptide PGLa

Experimental and Computational Studies Investigating Trehalose Protection of HepG2 Cells from Palmitate-Induced Toxicity Sukit Leekumjorn, Yifei Wu,

Volume 100, Issue 3, Pages (February 2011)

Reversible and Quantitative Photoregulation of Target Proteins

Pulmonary Surfactant Protein SP-C Counteracts the Deleterious Effects of Cholesterol on the Activity of Surfactant Films under Physiologically Relevant.

Paolo Mereghetti, Razif R. Gabdoulline, Rebecca C. Wade

The Complex Kinetics of Protein Folding in Wide Temperature Ranges

Volume 93, Issue 12, Pages (December 2007)

Carlos Mattea, Johan Qvist, Bertil Halle Biophysical Journal

Tapani Viitala, Jouko Peltonen Biophysical Journal

Congju Chen, Irina M. Russu Biophysical Journal

Mei Wang, Maggie Law, Jean Duhamel, P. Chen Biophysical Journal

Rheological Analysis and Measurement of Neutrophil Indentation

Volume 83, Issue 6, Pages (December 2002)

Effects of Monovalent Anions of the Hofmeister Series on DPPC Lipid Bilayers Part II: Modeling the Perpendicular and Lateral Equation-of-State E. Leontidis,

Volume 84, Issue 1, Pages (January 2003)

Protein Self-Association Induced by Macromolecular Crowding: A Quantitative Analysis by Magnetic Relaxation Dispersion Karim Snoussi, Bertil Halle Biophysical.

Ca2+ Regulation of Gelsolin Activity: Binding and Severing of F-actin

Jess V. Nauman, Phil G. Campbell, Frederick Lanni, John L. Anderson

Sergi Garcia-Manyes, Gerard Oncins, Fausto Sanz Biophysical Journal

Huan-Xiang Zhou, Osman Bilsel Biophysical Journal

Andreas Fibich, Karl Janko, Hans-Jürgen Apell Biophysical Journal

The Density and Refractive Index of Adsorbing Protein Layers

Main Phase Transitions in Supported Lipid Single-Bilayer

Paolo Mereghetti, Razif R. Gabdoulline, Rebecca C. Wade

Ana Coutinho, Liana Silva, Alexander Fedorov, Manuel Prieto

Presentation transcript:

Kinetic Analysis of Protein Crystal Nucleation in Gel Matrix Lei Wang, Xiang-Yang Liu Biophysical Journal Volume 95, Issue 12, Pages 5931-5940 (December 2008) DOI: 10.1529/biophysj.108.135574 Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 1 Temperature T1 and corresponding supersaturation (σ1) for lysozyme nucleation during the time Δt. Temperature T2 and corresponding supersaturation (σ2) for developing the crystals to detectable dimensions. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 2 Time dependence of the mean number of crystals per microliter at the supersaturation of 9.33 at 6°C. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 3 Effect of additives on the interfacial correlation factor f(m) and the nucleation kinetics. A promotion or inhibition effect will increase or decrease the interfacial correlation parameter in the plot of ln J ∼1/[ln(1+σ)]2. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 4 Protein 2D interface assembly kinetics. (a) The diffusion of protein molecules to the interface. (b) The penetration of protein molecules through the interface film from subsurface (a thickness of a few molecular diameters next to surface) to the surface (28). (c) Molecular rearrangements of adsorbed molecules in the film. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 5 Influence of agarose on the nucleation kinetics of lysozyme at pH=4.5, 6°C. Schematic plot of lnJ ∼ 1/[ln(1+σ)]2 with error bar. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 6 Schematic drawing of structural match between the nucleating phase and foreign bodies (a) in gel-free solution and (b) in gel solution. The gel fibers separate the foreign bodies and nuclei and lead to the structure mismatch between the two phases. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 7 Typical figure of surface tension of lysozyme solution at the air/water interface as a function of time at 6°Cfor selected values of supersaturation Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 8 ln(1−Π/Πe) as a function of time for lysozyme with and without 0.2% agarose. The rate constants for different steps can be obtained from the slope of the linear regression parts. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 9 Lysozyme crystals in gel-free (a) and gelled (b) 0.4μl microbatch drops under oil. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions

Figure 10 UV-Vis spectra of diluted lysozyme solution in the presence of 0.2% agarose gel and in the absence of agarose gel. Biophysical Journal 2008 95, 5931-5940DOI: (10.1529/biophysj.108.135574) Copyright © 2008 The Biophysical Society Terms and Conditions