Expression of basic fibroblast growth factor and its receptors in human ovarian follicles from adults and fetuses  Avi Ben-Haroush, M.D., M.Sc., Ronit Abir, Ph.D., Asangla Ao, Ph.D., Shaoguang Jin, M.D., Gania Kessler-Icekson, Ph.D., Dov Feldberg, M.D., Benjamin Fisch, M.D., Ph.D.  Fertility and Sterility  Volume 84, Pages 1257-1268 (October 2005) DOI: 10.1016/j.fertnstert.2005.05.018 Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 1 Immunocytochemical photographs of bFGF expression. (A) Ovarian section from a normal fetus at 20 GW. Note the positive staining (red-brown) for bFGF in the stromal cells and oocytes of primordial and primary follicles and negative staining (purple-blue) in the GCs. Original magnification ×200. (B) Ovarian section from a 19-year-old woman with osteosarcoma. Note the positive staining (red-brown) for bFGF in the stromal cells, partial cytoplasmic staining with nuclear staining in an oocyte of a primordial follicle, and negative staining (purple-blue) in the GCs. Original magnification ×200. (C) Negative control of the same ovary as in A (absorbed with the blocking peptide). Note the primordial follicle, the overall purple-blue staining, and the lack of red-brown staining. Original magnification ×200. Ben-Haroush. bFGF and its receptors in human ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1257-1268DOI: (10.1016/j.fertnstert.2005.05.018) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 2 Immunocytochemical photographs of FGFR-2 expression. (A) Ovarian section from a normal fetus at 20 GW. Note the positive staining (red-brown) in the oocytes of primordial and primary follicles and the negative staining (purple-blue) in the GCs. Original magnification ×200. (B) Ovarian sections from a 17-year-old girl with Hodgkin’s lymphoma. Note the positive staining in the oocytes and GCs of primordial (B1), primary (B2), and secondary (B3) follicles. Original magnification ×400. (C) Negative control of the same ovary as in B (absorbed with the blocking peptide). Note the secondary follicle, the overall purple-blue staining, and the lack of red-brown staining. Original magnification ×400. Ben-Haroush. bFGF and its receptors in human ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1257-1268DOI: (10.1016/j.fertnstert.2005.05.018) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 3 Immunocytochemical photographs of FGFR-3 expression. (A) Ovarian section from a normal fetus at 21 GW. Note the positive staining (red-brown) for FGFR-3 in the stromal cells and oocytes of primordial and primary follicles. Original magnification ×200. (B) Ovarian section from a 38-year-old woman with breast cancer. Note the positive staining in the GCs and light partial cytoplasmic staining in the oocyte of an antral follicle. Original magnification ×200. (C) Negative control of the same ovary as in A (absorbed with the blocking peptide). Note the secondary follicle, the overall purple-blue staining, and the lack of red-brown staining. Original magnification ×200. Ben-Haroush. bFGF and its receptors in human ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1257-1268DOI: (10.1016/j.fertnstert.2005.05.018) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 4 Immunocytochemical photographs of FGFR-4 expression. (A) Ovarian section from a fetus with achondroplasia at 33 GW. Note the positive staining (red-brown) for FGFR-4 in the oocyte of an antral follicle. Original magnification ×400. (B) Ovarian section from a 19-year-old woman with osteosarcoma. Note the positive staining (red-brown) in the oocytes of primordial and primary follicles, and in the GCs of the primary follicle. Original magnification ×400. (C) Negative control of the same ovary as in A (absorbed with the blocking peptide). Note the primordial follicles, overall purple-blue staining, and the lack of red-brown staining. Magnification ×200. Ben-Haroush. bFGF and its receptors in human ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1257-1268DOI: (10.1016/j.fertnstert.2005.05.018) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

FIGURE 5 Reverse transcription-polymerase chain reaction analysis was performed on adult and fetal samples. HPRT (hypoxanthine phosphoribosyl transferase) is a housekeeping gene used for positive control. F = fetus; W = woman. Ben-Haroush. bFGF and its receptors in human ovaries. Fertil Steril 2005. Fertility and Sterility 2005 84, 1257-1268DOI: (10.1016/j.fertnstert.2005.05.018) Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions