Wdr11 knockout mouse shows neuroendocrine and metabolic dysfunctions

Slides:

Advertisements

Similar presentations

Estrous Cycle Topic 3096 C Amanda Trutsch. Estrus The period of mating activity in the female mammal Same as heat.

Advertisements

Hormones of the female reproductive cycle: Anterior pituitary gland: Follicle stimulating hormone (FSH): Stimulates growth of ovarian follicles, and estrogen.

The Male Reproductive System

8.5: Reproductive Hormones Male Reproductive System Female Reproductive System.

Hormones That Affect Blood Sugar & Reproductive Hormones

Chapter 46: Animal Reproduction.

REPRODUCTIVE SYSTEM. Female Organs/structures Ovaries Uterus Fallopian tubes (oviduct) Vagina Cervix.

Chapter 46.4 and 46.5 Animal Reproduction.

9 The Endocrine System.

Role of TNF in fracture healing in vivo Addition of rhTNF at the fracture site on days 0 and 1 led to augmented healing, indicated by increased % callus.

Unit B: Reproduction and Development

Reproduction and Development

Loss of SORCS1 and SORCS3 increases hypothalamic expression of Agrp and Klf4 Loss of SORCS1 and SORCS3 increases hypothalamic expression of Agrp and Klf4.

Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.

Loss of Hdac3 impairs Ar signaling in mouse prostate tissues and has no effect on apoptosis Loss of Hdac3 impairs Ar signaling in mouse prostate tissues.

Hormonal Regulation of the Reproductive System

Reduced scar formation in the injured GM of CCR2−/− mice

Menstruation IF fertilization does NOT take place

AgRP and POMC Neurons Are Hypophysiotropic and Coordinately Regulate Multiple Endocrine Axes in a Larval Teleost Chao Zhang, Paul M. Forlano, Roger D.

THE OVARIES AND THE TESTES

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

PERK activator reduces behavioral deficits and neurodegeneration in vivo PERK activator reduces behavioral deficits and neurodegeneration in vivo A–CYoung.

Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice.

Volume 52, Issue 2, Pages (October 2006)

9 The Endocrine System.

Antiproliferative Role of Dopamine: Loss of D2 Receptors Causes Hormonal Dysfunction and Pituitary Hyperplasia Adolfo Saiardi, Yuri Bozzi, Ja-Hyun Baik,

Volume 26, Issue 2, Pages e4 (August 2017)

Human Reproductive System

RFGF21 induces neurite outgrowth in GnRH neurons in vitro, stimulates GnRH secretion ex vivo, and reaches GnRH neurons by fenestrated vessels in vivo rFGF21.

Volume 67, Issue 6, Pages e4 (September 2017)

No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO Cre+ mice No neurodegenerative phenotype observed in single Aph1a and Aph1bc cKO.

Volume 14, Issue 4, Pages (October 2011)

Effects of GM1 on astroglial and microglial markers

TFE3 prevents diet‐induced obesity and metabolic syndrome

Impaired glucose tolerance in adult S1/3 KO mice on a normal chow

Christophe Audouard, M.S., Elisabeth Christians, Ph.D.

Hormones & Reproduction

WDR11 cooperates with Hh pathway to regulate gene expression

Neuro‐inflammatory cytokines, brain microglial activation, and working memory deficits are normalized using LV.IDS.ApoEII, but not LV.IDS in MPS II mice.

Volume 2, Issue 6, Pages (December 2005)

Molecular and morphological analysis of Pitrm1+/− mouse brain

Intra‐eWAT administration of FGF21 vectors in ob/ob mice

Hdac3 deletion decreases AKT phosphorylation and tumor growth in Pten knockout prostate cancer Hdac3 deletion decreases AKT phosphorylation and tumor growth.

Rescuing mGPDH deficiency improves skeletal muscle regeneration during obesity and diabetes Rescuing mGPDH deficiency improves skeletal muscle regeneration.

Impaired ECM protein synthesis in infarcted area of S100a4cre+/− × Fstl1flox/flox mice Impaired ECM protein synthesis in infarcted area of S100a4cre+/−

Disease‐associated human WDR11 mutations are functionally defective

ER stress response and susceptibility to apoptosis are regulated by TFEB and TFE3 ER stress response and susceptibility to apoptosis are regulated by TFEB.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

mGPDH is not essential to muscle development

Co‐expression of Sorcs1 and Sorcs3 in the hypothalamus

Volume 19, Issue 1, Pages (July 1997)

Volume 12, Issue 3, Pages (September 2010)

I.c.v.‐injected AβOs induce increased food intake, hypothalamic expression of orexigenic neuropeptides but no hypothalamic cell degeneration AAccumulated.

Regulation of the Reproductive System

9 The Endocrine System.

Therapeutic role of TR in HFD‐established diabetic mice

The CHCH domain is necessary for mitochondrial import of CHCHD10

Lipid presentation by CD11c+ cells controls iNKT cell subsets

WDR11 is required for ciliogenesis

Treatment with AZD5363 reduces the proportion of proliferating vascular endothelial cells and following RT, the influx of CD11b+ bone marrow‐derived cells.

Catalytic activity of mTERT is needed for increased healthspan and life‐span extension in AAV9‐mTERT treated mice.A.Scheme of mTERT‐wt and mTERT‐DN, a.

GM1 decreases neuropathology and weight loss in R6/2 mice

Volume 12, Issue 3, Pages (September 2010)

Supplementary Figure 5: Effect of S48168 on normalized organ and skeletal muscle weights after 12 weeks of treatment for all the experimental groups from.

Robo3 in the GnRH neuronal system.

Kmt2b is required for SSC-to-progenitor transition.

PTZ-induced neuronal activity visualized by IEGs

SORCS1 and SORCS3 control energy balance and orexigenic peptide production Representative hematoxylin and eosin (H&E)‐stained sections of perigonadal white.

PC loss in Nhe6-null mouse cerebellum, female and male.

Volume 19, Issue 1, Pages (July 1997)

Presentation transcript:

Wdr11 knockout mouse shows neuroendocrine and metabolic dysfunctions Wdr11 knockout mouse shows neuroendocrine and metabolic dysfunctions Total numbers of GnRH neurons determined by counting the positive immunoreactivity in every fourth section of serially sectioned whole embryo head at E12.5 (WT, n = 5; Wdr11−/−, n = 5). Data are presented as means ± SEM. Statistical analysis by unpaired Student's t‐test (****P < 0.0001).Sagittal sections of the brain at E12.5–E14.5 and coronal sections at E18.5 were imaged after H/E staining to investigate pituitary development. rp, Rathke's pouch; vd, ventral diencephalon; m, mesenchyme; inf, infundibulum; rt, rostral tip; al, anterior lobe; il, intermediate lobe; hy, hypothalamus; 3v, third ventricle. Scale bars, 100 μm.Quantitative RT–PCR analyses of genes expressed in the hypothalamus and pituitary of 8‐ to 10‐week‐old males (n = 5) and dioestrous females (n = 5). Data are presented as means ± SEM. Statistical analysis by unpaired Student's t‐test (ns, P > 0.05; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). GnRHR, GnRH receptor; LH, luteinizing hormone; FSH, follicle‐stimulating hormone; GH, growth hormone; PRL, prolactin.Numbers of pups per litter produced after mating with WT B6 females were tracked in one WT and two null male mice for a period of 7 months. One‐way ANOVA with Dunnett's post hoc test indicates significantly reduced fertility in the null (***P < 0.001; ****P < 0.0001). Data presented as means ± SEM.A photograph showing the male testes. H/E‐stained sections of testes show hypoplastic Leydig cells and vacuolated seminiferous tubules (asterisk) containing few spermatozoa and spermatids in Wdr11−/− (Scale bars, 100 μm). Phase‐contrast images of the sperm show morphological abnormalities in the null (Scale bars, 20 μm).A photograph showing the female uteri. H/E‐stained sections of the uterine wall and ovaries are shown. FL, follicle; CL, corpus luteum; HC, haemorrhagic cyst. Scale bars, 500 μm.Body length (BL) and weight (BW) were measured, and body mass index (BW/BL, g/cm2) was calculated from the measurements at 1 week (WT, n = 6; Wdr11+/−, n = 6; Wdr11−/−, n = 6) and 25 weeks (WT, n = 8; Wdr11+/−, n = 8; Wdr11−/−, n = 6). Body fat percentages of WT and Wdr11+/− mice at 25 weeks were calculated by dividing fat weight by body weight. Data are presented as means ± SEM. Statistical analysis by unpaired Student's t‐test (ns, P > 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001). Source data are available online for this figure. Yeon‐Joo Kim et al. EMBO Rep. 2018;19:269-289 © as stated in the article, figure or figure legend