Volume 12, Issue 23, Pages 1992-2001 (December 2002) Genomic Scale Mutant Hunt Identifies Cell Size Homeostasis Genes in S. cerevisiae  Jian Zhang, Colette Schneider, Lisa Ottmers, Robert Rodriguez, Audra Day, Jody Markwardt, Brandt L. Schneider  Current Biology  Volume 12, Issue 23, Pages 1992-2001 (December 2002) DOI: 10.1016/S0960-9822(02)01305-2

Figure 1 Systematic Cell Size Analysis of Yeast Deletion Strains Grown to Saturation (A) Statistical determination of whi and uge mutants. The cell size data from 5553 individual measurements was used to calculate the average mean, median, and mode cell sizes. Standard deviations are also shown. For reference, the average mean, median, and mode cell sizes with their standard deviations of 40 independent wild-type colonies are shown. (B) Cell size distributions of 5553 yeast strain deletions. For simplicity, the total number of mutants in a 5 fL window of size is plotted versus cell volume. The three curves representing the mean (circles), median (squares), and mode (triangles) cell sizes are symmetrical and nearly completely superimposable, with 95% of mutants having cell sizes between 39.6 fL and 66.0 fL (±2 SD of the average median cell size). (C) Cell size curves of a whi mutant, ada1, an uge mutant, spt10, and wild-type cells are shown. Current Biology 2002 12, 1992-2001DOI: (10.1016/S0960-9822(02)01305-2)

Figure 2 Cell Size Is Regulated by Changes in “Critical Cell Size” (A) Plots of the percentage of budded cells as a function of cell size: wild-type cells (circles) and three small cell mutants, mck1 (squares), rip1 (triangles), and sac1 (diamonds). These data show that each of these mutants become 50% budded at ∼55 fL, as compared to ∼65 fL for wild-type cells. This reduction in “critical cell size” is consistent with that observed in other previously identified whi mutants or through the overexpression of CLN genes. (B) Plots of the percentage of budded cells as a function of cell size: wild-type cells (circles) and three uge mutants, cln3 (squares), met32 (triangles), and rpt2 (diamonds). These data show that each of these mutants become 50% budded at ∼80 fL (met32), ∼115 fL (cln3), and ∼120 fL (rpt2), as compared to ∼65 fL for wild-type cells. These data suggest that cell size is regulated primarily through changes in “critical cell size.” Current Biology 2002 12, 1992-2001DOI: (10.1016/S0960-9822(02)01305-2)