Chemokine profile of synovial fluid from normal, osteoarthritis and rheumatoid arthritis patients: CCL25, CXCL10 and XCL1 recruit human subchondral mesenchymal.

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Volume 94, Issue 2, Pages (March 2014)

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Chemokine profile of synovial fluid from normal, osteoarthritis and rheumatoid arthritis patients: CCL25, CXCL10 and XCL1 recruit human subchondral mesenchymal progenitor cells M. Endres, K. Andreas, G. Kalwitz, U. Freymann, K. Neumann, J. Ringe, M. Sittinger, T. Häupl, C. Kaps Osteoarthritis and Cartilage Volume 18, Issue 11, Pages 1458-1466 (November 2010) DOI: 10.1016/j.joca.2010.08.003 Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Chemokine profile of human SF. Representative chemokine antibody array membranes showing the chemokine profile of SF derived from normal (A), OA (B) and RA (C) donors. The respective chemokines were measured in duplicates per array (D). In total, per group n=5 SF were analyzed. Note that levels of a given chemokine can be compared from array to array, but not the levels of one chemokine to the other. Positive controls on the array membrane are streptavidin conjugated horseradish peroxidase and negative controls are bovine serum albumins. Osteoarthritis and Cartilage 2010 18, 1458-1466DOI: (10.1016/j.joca.2010.08.003) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Gene expression analysis of human progenitor cells for selected chemokine receptors. Real-time PCR analysis (n=9) of three independent CSP preparations (passage three) showed a low level of expression of CXCR1-4 and CXCR7 as well as CCR2, CCR3, CCR9, CCR10 and XCR1. CCR4 was not detected. The mean of each expression level is plotted and the error bars represent 95% CI (lower/upper limit). Osteoarthritis and Cartilage 2010 18, 1458-1466DOI: (10.1016/j.joca.2010.08.003) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Immuno-histochemical staining of chemokine receptors on human progenitor cells. Immuno-histochemical staining of CXCR3, CCR9 and XCR1 showed presence of the respective receptors on human subchondral progenitor cells on the protein level. Controls gave no signal and show specificity of the antibody staining. Osteoarthritis and Cartilage 2010 18, 1458-1466DOI: (10.1016/j.joca.2010.08.003) Copyright © 2010 Osteoarthritis Research Society International Terms and Conditions