Shigetoshi Mieno, MD, Richard T

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Presentation transcript:

Characteristics and Function of Cryopreserved Bone Marrow–Derived Endothelial Progenitor Cells Shigetoshi Mieno, MD, Richard T. Clements, PhD, Munir Boodhwani, MD, Neel R. Sodha, MD, Basel Ramlawi, MD, Cesario Bianchi, MD, PhD, Frank W. Sellke, MD The Annals of Thoracic Surgery Volume 85, Issue 4, Pages 1361-1366 (April 2008) DOI: 10.1016/j.athoracsur.2007.12.006 Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Direct fluorescent staining of endothelial progenitor cells (EPC) from fresh mononuclear cells (MNCs) (I) and cryopreserved mononuclear cells (II). (A) Isolectin B4 staining. (B) Acetylated low-density lipoprotein labeled with 1,1′-dioctadecyl-3,3,3′-3′-tetramethylindo-carbocyanine perchlorate staining. (C) 4′,6-diamidino-2-phenylindole dihydrochloride staining. (D) Merged images of all three staining techniques. The Annals of Thoracic Surgery 2008 85, 1361-1366DOI: (10.1016/j.athoracsur.2007.12.006) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Proliferation activity in cell culture media. White column, endothelial progenitor cells from fresh mononuclear cells; black column, endothelial progenitor cells from cryopreserved mononuclear cells. Asterisk and sharp indicate significance compared with white and black columns of control, respectively. The Annals of Thoracic Surgery 2008 85, 1361-1366DOI: (10.1016/j.athoracsur.2007.12.006) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Vascular endothelial growth factor (VEGF)–induced proliferation activity of endothelial progenitor cells. White column, endothelial progenitor cells from fresh mononuclear cells; black column, endothelial progenitor cells from cryopreserved mononuclear cells. Asterisk and sharp indicate significance compared with white and black column of control, respectively. The Annals of Thoracic Surgery 2008 85, 1361-1366DOI: (10.1016/j.athoracsur.2007.12.006) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Migration activity in response to stromal-derived factor-1α (SDF-1α). White column, endothelial progenitor cells from fresh mononuclear cells; black column, endothelial progenitor cells from cryopreserved mononuclear cells Asterisk and sharp indicate significance compared with white and black column of control, respectively. The Annals of Thoracic Surgery 2008 85, 1361-1366DOI: (10.1016/j.athoracsur.2007.12.006) Copyright © 2008 The Society of Thoracic Surgeons Terms and Conditions