ZBTB48 associates with short and long telomeres in vivo

Slides:

Advertisements

Similar presentations

The expressions of H19 and Igf1r are significantly decreased in the eutopic endometrium of women with endometriosis compared to those without endometriosis.

Advertisements

Additional File 12. HeLa barcode passaging experimental results.

Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence Overexpression of PAI‐1 reverses the arsenite‐mediated effect on senescence.

Cycloheximide inhibits arsenite‐induced stress granule formation and prevents the decreased number of senescent cells mediated by repeated arsenite treatment.

Morphology of white adipose tissue and muscle in S1/3 KO mice of different ages Morphology of white adipose tissue and muscle in S1/3 KO mice of different.

Reduced astrocyte proliferation results in increased immune cell infiltration into the injured GM Reduced astrocyte proliferation results in increased.

A culture‐induced TE‐like subpopulation

JNK is activated by Wnt5a.

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

(A) Left panel: rate of H2O2 release per minute was analysed by calculating the linear slope of data obtained in Supplementary Figure S1A. (A) Left panel:

Measurement of terminal restriction fragments (TRFs) in bone marrow cells from both parents and the G4 progeny. Measurement of terminal restriction fragments.

Wild‐type p53 binds and represses Rad51 promoter.

TRF2/RAP1complex mediates C‐NHEJ inhibition at telomeric ends through hindrance at the KU loading and DNA–PK activation steps. TRF2/RAP1complex mediates.

Clonal expansion of sporadically induced K‐rasG12D in Lgr5hi cells Confocal scanning of the bottom of small intestinal crypts at indicated time points.

Volume 38, Issue 4, Pages (May 2010)

Control of IL‐6 synthesis by ζPKC and RelA Ser311 phosphorylation.

NoRC regulates telomeric heterochromatin.

Basal autophagic flux and peroxisome abundance are not affected in USP30 KO cells Basal autophagic flux and peroxisome abundance are not affected in USP30.

Both NM1 and the WSTF complex stimulate Pol I transcription.

NoRC affects centromere function.

HS induces sustained H3K4me3 and H3K4me2 methylation at HSP22

Characterization of promoters relative to pAGA1

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)‏ The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)

Supplemental Figure 4 A B TRF1 PAR siGFP TRF2DBDC siGFP TRF2DBDC

Impaired glucose tolerance in adult S1/3 KO mice on a normal chow

Widespread modulation of epigenetic landscape for differentiated organoids is driven by Hnf4g Widespread modulation of epigenetic landscape for differentiated.

Expression and occupancy of a set of transcription factors corresponding to the identified motifs at FAIRE peaks Expression and occupancy of a set of transcription.

MARCH6 and TRC8 degrade membrane‐associated mCherry‐CL1

Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi mutant Expression of GFP‐Aub transgenes in GSCs, and GSC loss phenotype in armi.

Effect of HUWE1 on MYC and MIZ1 complexes Immunoblot documenting levels of MYC and MIZ1 upon HUWE1 overexpression. Effect of HUWE1 on MYC and MIZ1 complexes.

Endogenous MOSPD2 is recruited to interorganelle contact sites by FFAT‐containing proteins Endogenous MOSPD2 is recruited to interorganelle contact sites.

FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL FFAT motif‐dependent recruitment of MOSPD2 in ER–endosome contacts by STARD3NL.

Mapping of small RNAs to LINE‐1 sequence (related to Figs 3 and 4)‏

Zc3h10 overexpression boosts mitochondrial function and density in myotubes Zc3h10 overexpression boosts mitochondrial function and density in myotubes.

Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation Zc3h10 downregulation impairs mitochondrial function and C2C12 differentiation.

System approaches to analyze the effects of Zc3h10 knockdown

Analysis of deep‐sequencing data obtained from the libraries of MILI‐bound piRNAs and total small RNAs (related to Fig 4)‏ Analysis of deep‐sequencing.

Lack of GTSF1 causes defects in MILI‐directed piRNA amplification

Deficient segregation of the lac operator insertion.

Tsc1 co‐chaperone inhibits Hsp90 chaperone function

Ssu72 is required for telomeric C‐strand stability

Example of processed data visualized with “Peak Browser” of ChIP‐Atlas

Loss of ANAC032 increases disease susceptibility to Pst DC3000

Characterization of super‐enhancers in WT and KO macrophages

HMGB1 reverses mitochondrial DNA damage caused by mutant Atxn1 The mitochondrial DNA amplification assay with cerebellar tissue revealed that mitochondrial.

STARD3 favors cholesterol accumulation in endosomes

AUP, UBE2G2 and TRC8 facilitate the degradation of mCherry‐CL1 through a shared pathway AUP, UBE2G2 and TRC8 facilitate the degradation of mCherry‐CL1.

The DNA Damage Machinery and Homologous Recombination Pathway Act Consecutively to Protect Human Telomeres Ramiro E. Verdun, Jan Karlseder Cell Volume.

NDP52 promotes CHIKV infection in a species‐specific manner.

Dynamics of induction of mating promoters after pheromone stimulation

NoRC suppresses recombination between telomeric repeats.

Cbl protein levels in ago3, twin, and armi mutant GSCs

Validation of cell types and functional validation of KAP1 knockout

Examples of analysis with “Target Genes” and “Colocalization” of ChIP‐Atlas Examples of analysis with “Target Genes” and “Colocalization” of ChIP‐Atlas.

Examples of “Enrichment Analysis”

Impact of RTEL1 variants on telomere maintenance.

Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling. Protein tyrosine kinase 7 is involved in Wnt/β‐catenin canonical signalling.

A near‐haploid genetic screen identifies genes required for cytosolic protein quality control in human cells A near‐haploid genetic screen identifies genes.

ZBTB48 is required for MTFP1 expression

The CHCH domain is necessary for mitochondrial import of CHCHD10

Functional metabolic rearrangements in chloramphenicol‐resistant populations Functional metabolic rearrangements in chloramphenicol‐resistant populations.

Overview of the ChIP‐Atlas data set and computational processing

LIR‐mediated cross talk between cluster formation and the autophagy machinery LIR‐mediated cross talk between cluster formation and the autophagy machinery.

TRAF4 is required for EGFR activation in response to EGF stimulation.

Web pages for integrative analyses in ChIP‐Atlas

GATC sites where methylation changes over time correspond to a change in gene expression in a dam mutant. GATC sites where methylation changes over time.

Fig. 1. Mitotic arrest results in differential RNA association with coilin.Untreated or nocodazole treated HeLa cell lysate was used for RNA immunoprecipitations.

Increased anti-DNA Abs in 2KO-Bcl6TC mice.

Influence of RdDM on DCL4 Transcript Isoform Expression.

Presentation transcript:

ZBTB48 associates with short and long telomeres in vivo ZBTB48 associates with short and long telomeres in vivo TTAGGG content of telomeric reads in ChIPseq samples from U2OS WT, U2OS ZBTB48 KO and U2OS HOT1 KO clones using IgG, TRF2, ZBTB48 Atlas, ZBTB48 GeneTex and HOT1 antibodies as well as FLAG antibody for FLAG‐ZBTB48 WT and point mutants for ZnF10 and ZnF11. The percentage contribution to all reads containing 1–8× TTAGGG repeats is shown. All reactions were performed in technical replicates on two independent WT and KO clones each.MEME sequence logo and bit score for the top 500 extratelomeric ChIPseq peaks for ZBTB48 Atlas, ZBTB48 GeneTex, TRF2 and HOT1 antibodies compared to IgG samples in U2OS. For each antibody, the most frequent motif is shown.TTAGGG enrichment in ChIPseq samples with reads with 7× or 8× TTAGGG repeats. Absolute read counts from ChIPseq reactions from each two HeLa and U2OS WT and KO clones with two independent antibodies for ZBTB48 and HOT1 are shown (left). The corresponding fold enrichments are calculated (right) and compared to TRF2 ChIPseq reactions for which fold enrichments are calculated relative to IgG samples. Error bars represent standard deviations (n = 4), and P‐values are based on Student's t‐test with ** indicating P < 0.01. Arne Jahn et al. EMBO Rep. 2017;embr.201744095 © as stated in the article, figure or figure legend