The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova,

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Presentation transcript:

The Development of a Multitarget, Multicolor Fluorescence in Situ Hybridization Assay for the Detection of Urothelial Carcinoma in Urine Irina A. Sokolova, Kevin C. Halling, Robert B. Jenkins, Haleh M. Burkhardt, Reid G. Meyer, Steven A. Seelig, Walter King The Journal of Molecular Diagnostics Volume 2, Issue 3, Pages 116-123 (August 2000) DOI: 10.1016/S1525-1578(10)60625-3 Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 A and B demonstrate that cells with FISH abnormalities frequently exhibit nuclear abnormalities and that these nuclear abnormalities can be used to facilitate scanning for FISH abnormal cells. Arrows in A point to abnormal nuclei as seen with DAPI stain only. Arrows in Bpoint to the same abnormal nuclei and reveal that these cells have multiple copies of chromosomes 3 (red signals) and 7 (green signals). The Journal of Molecular Diagnostics 2000 2, 116-123DOI: (10.1016/S1525-1578(10)60625-3) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Examples of FISH normal and abnormal cases with optimized probe set.A shows an example of a FISH normal cell from a normal urine donor. B shows an example of a FISH abnormal cell with polysomy from a patient with biopsy-proven UC after FISH. CEP7 (green), CEP3 (red), CEP17 (aqua), and LSI 9p21 (yellow). The Journal of Molecular Diagnostics 2000 2, 116-123DOI: (10.1016/S1525-1578(10)60625-3) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Receiver-operator characteristic curve showing the sensitivity and specificity of the FISH assay for UC detection based on the number of cells with polysomy. This curve was determined from 46 patients with biopsy-proven UC (sensitivity) and 63 patients without evidence of bladder cancer (specificity). A cutoff of five or more cells with polysomy provided a sensitivity of 84.2% and specificity of 91.8%. The Journal of Molecular Diagnostics 2000 2, 116-123DOI: (10.1016/S1525-1578(10)60625-3) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

The Journal of Molecular Diagnostics 2000 2, 116-123DOI: (10 The Journal of Molecular Diagnostics 2000 2, 116-123DOI: (10.1016/S1525-1578(10)60625-3) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions