Gut environment context affects C

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Gut environment context affects C Gut environment context affects C. difficile sporulation and toxin activity. Gut environment context affects C. difficile sporulation and toxin activity. Quantification of spore CFU and toxin titer from cecal content of infected mice (n = 9 per group). (A) Vegetative C. difficile CFU per gram of cecal content (P > 0.05). (B) C. difficile spore CFU per gram of cecal content. (C) Toxin titer from cecal content measured by activity in Vero cell rounding assay. Dotted lines denote limits of detection (LOD). Values for undetectable points were imputed as half the LOD for calculation of significant differences. Significance (P < 0.05), denoted by a single asterisk, was determined with the Wilcoxon signed-rank test with the Benjamini-Hochberg correction. Matthew L. Jenior et al. mSystems 2017; doi:10.1128/mSystems.00063-17