H3 Dynamics in Wild-Type and fas1-4 Mutant Roots. H3 Dynamics in Wild-Type and fas1-4 Mutant Roots. (A) to (D) Images of the root epidermal cell layer of H3.1(HTR3)-GFP ([A] and [B]) and H3.3(HTR4)-GFP ([C] and [D]) expressed in wild-type ([A] and [C]) and fas1-4 mutant background ([B] and [D]). Insets show a magnification of nuclear labeling patterns. Note the decrease of the HTR3-GFP signal (B) and the enrichment of H3.3(HTR4)-GFP in the chromocenters (D) in fas1-4 plants. (E) The H3.1/H3.3 balance along the root developmental zones in fas1-4 mutant plants. Measurements were carried as described for Figure 4F. Sofía Otero et al. Plant Cell 2016;28:1361-1371 ©2016 by American Society of Plant Biologists