Expression of forkhead transcription factors in human granulosa cells

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Expression of forkhead transcription factors in human granulosa cells Margareta D. Pisarska, M.D., Fang-Ting Kuo, Ph.D., Dahai Tang, Ph.D., Parham Zarrini, Salma Khan, M.D., Ph.D., Aline Ketefian, M.D. Fertility and Sterility Volume 91, Issue 4, Pages 1392-1394 (April 2009) DOI: 10.1016/j.fertnstert.2008.04.054 Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions

Figure 1 Expression of FOXO1, FOXO3, and FOXO4 in human luteinized mural granulosa cells. Total RNA isolated from human granulosa cells taken from ten different patients were reverse-transcribed and polymerase chain reaction (PCR) amplified with gene-specific primer pairs. Human luteinized mural granulosa cells from all ten patients were positive for the 170-bp product of FOXO1 (a), the 186-bp product of FOXO3 (b), and the 173-bp product of FOXO4 (c), as well as for β-actin (d). M = molecular weight marker; + = positive control; − = negative control. Fertility and Sterility 2009 91, 1392-1394DOI: (10.1016/j.fertnstert.2008.04.054) Copyright © 2009 American Society for Reproductive Medicine Terms and Conditions