Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight.

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Presentation transcript:

Species identification of Aspergillus, Fusarium and Mucorales with direct surface analysis by matrix-assisted laser desorption ionization time-of-flight mass spectrometry E. De Carolis, B. Posteraro, C. Lass-Flörl, A. Vella, A.R. Florio, R. Torelli, C. Girmenia, C. Colozza, A.M. Tortorano, M. Sanguinetti, G. Fadda Clinical Microbiology and Infection Volume 18, Issue 5, Pages 475-484 (May 2012) DOI: 10.1111/j.1469-0691.2011.03599.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Three-dimensional principal component analysis (PCA) plot of the technical replicates of selected reference strains of (a) Aspergillus (seven strains), (b) Fusarium (four strains) and (c) Mucorales (four strains). Every dot represents a single technical replicate. The spectra of a particular fungal reference strain are colour-coded: (a) Emericella nidulans UCSC401 in green, Aspergillus niger FL6 in yellow, Aspergillus terreus FL64 in blue, Aspergillus tubingensis CBS115.29 in dark pink, Aspergillus oryzae CBS819.72 in light blue, Aspergillus lentulus CBS117887 in brown and Aspergillus fumigatus CG221 in red; (b) Fusarium solani species complex UCSC490 in red, Fusarium dimerum CBS366.73 in green, Gibberella fujikuroi species complex UCSC464 in yellow and Fusarium oxysporum species complex UCSC512 in blue; and (c) Mucor circinelloides FL84 in red, Lichtheimia corymbifera FL41 in green, Rhizopus microsporus FL15 in blue and Rhizopus oryzae UCSC74 in yellow. Clinical Microbiology and Infection 2012 18, 475-484DOI: (10.1111/j.1469-0691.2011.03599.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Cluster analysis of matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of selected reference strains and challenge isolates (CH15, CH25 and CH51) identified as Aspergillus section Flavi species. Distance is displayed in relative units. Clinical Microbiology and Infection 2012 18, 475-484DOI: (10.1111/j.1469-0691.2011.03599.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Cluster analysis of matrix-assisted laser desorption ionization time-of-flight mass spectrometry spectra of all of the reference strains and challenge isolates (CH65 and CH70) of Fusarium solani species complex strains studied. Distance is displayed in relative units. ST, sequence type. Clinical Microbiology and Infection 2012 18, 475-484DOI: (10.1111/j.1469-0691.2011.03599.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions