Questionnaire & physical exam

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Questionnaire & physical exam Timeline Questionnaire & physical exam Serum Dust sample Blood RNA Blood DNA Stool Meconiun & Mother n = 326 n = 200 Newborns n = 326** n = 71** n = 90 Child (3-6 months) n = 286* n = 78 n = 272‡ n = 245 n = 45 (12 months) n = 280 n = 205 n = 9 n = 272 n = 178 n = 57 (24 months) n = 274 n = 169 n = 82 n = 153 n = 51 Measurements & Analyses Demographics, exposures & clinical outcomes * (x2) validation of lifestyle + follow-up sIgE, IgA IgG levels **from cord blood Allergens & endotoxin levels ‡House floors (n = 232) child’s bed (n = 347) Gene expression Genotyping & re-seq Parasite analysis & Kato Katz Molecular analysis of gut microflora Figure 3. Timeline of data and sample collection activities in the cohort from 0 to 24 months