Volume 44, Issue 5, Pages 947-955 (May 2006) The finest branches of the biliary tree might induce biliary vascularization necessary for biliary regeneration Marius C. van den Heuvel, Annette S.H. Gouw, Marcel Boot, Maarten J.H. Slooff, Sibrand Poppema, Koert P. de Jong Journal of Hepatology Volume 44, Issue 5, Pages 947-955 (May 2006) DOI: 10.1016/j.jhep.2005.08.011 Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 1 Portal tract of a control patient at reperfusion (A), 1-week (B) and 1-year (C). Biliary epithelium is stained in red, endothelium is stained in brown. (A) and (C): normal bile ducts (black arrow) and bile ductules (black arrowheads) with escorting microvasculature. (B): damaged bile duct (black arrow). Bile ductules without escorting microvessels (white arrowheads) are increased. Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 2 Portal tract of a CR patient at reperfusion (A), 1-month (B) and 1-year (C). Biliary epithelium is stained in red, endothelium is stained in brown. (A): normal bile ducts (black arrow) and bile ductules (black arrowheads) with escorting microvasculature. (B): bile duct (white arrow) and only two bile ductules (white arrowheads) without escorting microvasculature. (C): damaged bile ducts without escorting microvessels (white arrows). Absence of bile ductules. Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 3 Portal tract of a control patient at reperfusion (A) and 1-week (B). (A): bile duct (black arrow) and bile ductules (black arrowheads) showing a weak positive staining for VEGF-A. (B): bile duct (black arrow) and bile ductules (black arrowheads) in the control group at 1-week showing strong positive staining for VEGF-A In both figures hepatocytes show a weak membranous expression. Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 4 Boxplots showing numbers of bile ducts with capillaries (A), without capillaries (B) and total number of bile ducts (C) per portal tract at reperfusion (RPB), 1-week (1-W), 1-month (1-M) and 1-year (1-Y) in CR and the controls. (A): *, lower in CR at 1-year (reperfusion P=0.012; 1-week P=0.021). Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 5 Boxplots showing numbers of bile ductules with microvessels (A), without microvessels (B) and the total number of bile ductules (C) per portal tract at reperfusion (RPB), 1-week (1-W), 1-month (1-M) and 1-year (1-Y) in CR and the controls. (A): *, lower in CR at 1-year (reperfusion P=0.004; 1-week P=0.005; 1-month P=0.028); #, higher in controls at 1-month (reperfusion P=0.049; 1-year P=0.007). (B): #, higher in controls at 1-week (reperfusion P=0.003; 1-month P=0.036; 1-year P=0.001); ##, higher in controls at 1-month (reperfusion P=0.011; 1-year P=0.002). (C): *, lower in CR at 1-year (reperfusion P=0.006; 1-week P=0.007; 1-month P=0.015); #, higher in controls at 1-week (reperfusion P=0.026; 1-year P=0.008); ##, higher in controls at 1-month (reperfusion P=0.017; 1-year P=0.003). Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 6 Boxplots showing numbers of extraportal biliary cells with microvessels (A), without microvessels (B) and the total number of extraportal biliary cells (C) per portal tract at reperfusion (RPB), 1-week (1-W), 1-month (1-M) and 1-year (1-Y) in CR and the controls. (A): *, higher in CR at reperfusion (1-week P=0.01; 1-year P=0.003); **, higher in CR at 1-month (1-year P=0.038). (C): *, higher in CR at reperfusion (1-week P=0.05; 1-year P=0.006); **, higher in CR at 1-month (1-year P=0.041). Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 7 Boxplots showing the total portal (A) and extraportal (B) microvascular compartment. (A): #, lower in controls at 1-week (reperfusion P=0.006); ##, higher in controls at 1-month (reperfusion P=0.003; 1-week P=0.03); ###, lower in controls at 1-year (1-month P=0.02). (B): *, higher in CR at reperfusion (1-week P=0.034; 1-month P=0.005); #, higher in controls at reperfusion (1-week P=0.002; 1-month P<0.001); ##, higher in controls at 1-year (1-month P=0.007). Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 8 Line graphs representing the median values of the counted portal structures with exception of bile ducts per portal tract in time. Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions
Fig. 9 Line graphs representing the median values of the extraportal counted structures per portal tract in time. Journal of Hepatology 2006 44, 947-955DOI: (10.1016/j.jhep.2005.08.011) Copyright © 2005 European Association for the Study of the Liver Terms and Conditions