France - 15% on cured leaf; 10% on plants for breeder’s seed Selection level France - 15% on cured leaf; 10% on plants for breeder’s seed Japan – 5% on plants for seed production USA - 15% on cured leaf from cultivar evaluation Zimbabwe – 10% on cured leaf; 3% on plants for seed production

Bale sampling Bales ~ 30 kg Core sampler to provide ~ 100 g Sampling may disturb air movement Temperature and RH monitoring for ambient and within bale NO and NO2 emissions into air space within bale Tobacco into the bale at 3 different moisture contents Measured – moisture, alkaloids, NO2, alkaloids, TSNA