DNA Hypermethylation of MAL: A Promising Diagnostic Biomarker for Colorectal Tumors Guro E. Lind, Terje Ahlquist, Ragnhild A. Lothe Gastroenterology Volume 132, Issue 4, Pages 1631-1632 (April 2007) DOI: 10.1053/j.gastro.2007.03.003 Copyright © 2007 AGA Institute Terms and Conditions
Figure 1 Representative methylation-specific polymerase chain reaction (MSP) results from the analysis of MAL in 3 normal mucosa samples, 3 adenomas, and 3 carcinomas. A visible PCR product in lanes U indicates the presence of unmethylated alleles whereas a PCR product in lanes M indicates the presence of methylated alleles. Abbreviations: A, adenoma; C, carcinoma, N normal mucosa; POS, positive control consisting of normal blood (control for unmethylated samples) and in vitro methylated DNA (control for methylated samples); NEG, negative control (containing water as template); U, lane for unmethylated MSP product; M, lane for methylated MSP product. The illustration is a merge of 2 gel panels as the adenomas were run on a separate gel. Gastroenterology 2007 132, 1631-1632DOI: (10.1053/j.gastro.2007.03.003) Copyright © 2007 AGA Institute Terms and Conditions