E. Galmozzi, F. Facchetti, R. Perbellini, A. Aghemo

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Presentation transcript:

High-resolution melting assay for genotyping of IFNL4-associated dinucleotide variant rs368234815 E. Galmozzi, F. Facchetti, R. Perbellini, A. Aghemo Clinical Microbiology and Infection Volume 20, Issue 11, Pages O936-O938 (November 2014) DOI: 10.1111/1469-0691.12637 Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Representative difference graph for DNA samples from hepatitis C virus (HCV) infected patients carrying different rs368234815 genotypes (TT/TT: red lines, TT/-G: blue lines and -G/-G: green lines), was achieved using the LightCycler® NanoSW1.1 software (Roche, Mannheim, Germany) by subtracting the normalized fluorescence data of the TT/TT genotype, selected as a baseline, from that of each of the other samples in the high-resolution melting analysis. The position of each sample relative to the baseline is plotted against the temperature to generate a difference plot that helps to cluster samples manually into groups that have similar melting curves (e.g., those with the same genotype). Clinical Microbiology and Infection 2014 20, O936-O938DOI: (10.1111/1469-0691.12637) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions