Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines

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Presentation transcript:

Comparison of the immunogenicity of BM32, a recombinant hypoallergenic B cell epitope–based grass pollen allergy vaccine with allergen extract–based vaccines Milena Weber, MSc, Katarzyna Niespodziana, PhD, Birgit Linhart, PhD, Angela Neubauer, PhD, Hans Huber, PhD, Rainer Henning, PhD, Rudolf Valenta, MD, Margarete Focke-Tejkl, PhD Journal of Allergy and Clinical Immunology Volume 140, Issue 5, Pages 1433-1436.e6 (November 2017) DOI: 10.1016/j.jaci.2017.03.048 Copyright © 2017 The Authors Terms and Conditions

Fig 1 Induction of grass pollen allergen–specific IgG responses in rabbits by means of immunization with BM32, recombinant grass pollen allergens, and natural grass pollen AIT extracts. A, Scheme of rabbit immunizations. Rabbits were immunized with 3 monthly injections of BM32 or a mix of recombinant grass pollen allergens. In parallel, rabbits were immunized with registered grass pollen extract–based AIT vaccines (Allergovit, Alutard, Phostal, and Pollinex), as recommended by the manufacturer. Serum samples were collected from all rabbits shortly before the first immunization (Pre) and in monthly intervals (1.IS, 2.IS, 3.IS, and 4.IS). B, Comparisons and time courses of allergen-specific IgG responses of rabbits. Shown are mean OD values corresponding to allergen-specific IgG levels (y-axes) at different time points (x-axes). s.c, Subcutaneous. Journal of Allergy and Clinical Immunology 2017 140, 1433-1436.e6DOI: (10.1016/j.jaci.2017.03.048) Copyright © 2017 The Authors Terms and Conditions

Fig 2 Inhibition of IgE binding in allergic patients to grass pollen allergens with rabbit antibodies induced by vaccination. Shown are the percentages of inhibition of IgE binding (y-axes) to timothy grass pollen extract, Phl p 1, Phl p 2, Phl p 5, and Phl p 6 by anti-Allergovit, anti-Alutard, anti-Phostal, anti-Pollinex, or anti-BM32 rabbit antibodies (x-axes). Significant differences between BM- and extract-induced antibodies regarding inhibition of IgE binding are indicated. *P < .05, **P < .01, and ***P < .001. ns, Nonsignificant. Journal of Allergy and Clinical Immunology 2017 140, 1433-1436.e6DOI: (10.1016/j.jaci.2017.03.048) Copyright © 2017 The Authors Terms and Conditions

Fig E1 Boosting of BM32-induced allergen-specific IgG antibody responses. A, Scheme and time course of boosting experiment. Indicated are the time course (x-axis), injections, and dates of serum collection. B, Kinetics of allergen-specific IgG responses in BM32-immunized rabbits after initial immunization and 2 booster injections. Shown are mean OD values corresponding to allergen-specific IgG levels (y-axes) at different time points (x-axes). Journal of Allergy and Clinical Immunology 2017 140, 1433-1436.e6DOI: (10.1016/j.jaci.2017.03.048) Copyright © 2017 The Authors Terms and Conditions

Fig E2 Cross-reactivity of BM32-induced antibodies with pollen extracts from different grasses. Nitrocellulose-blotted pollen extracts from Phleum pratense, Lolium perenne, Cynodon dactylon, and Secale cereale were reacted with sera obtained from rabbits immunized with Allergovit, Alutard, Pollinex, Phostal, or BM32 before immunization (P) and after 4 monthly intervals (1, 2, 3, and 4). Bound rabbit IgG was detected with iodine 125–labeled antibodies and visualized by means of autoradiography. Molecular weights (in kilodaltons) are indicated on the left margins. Journal of Allergy and Clinical Immunology 2017 140, 1433-1436.e6DOI: (10.1016/j.jaci.2017.03.048) Copyright © 2017 The Authors Terms and Conditions

Fig E3 Rabbit antibodies induced by means of vaccination with BM32 and registered allergen extract–based AIT vaccines inhibit allergen-specific T-cell proliferation. PBMCs from a patient with grass pollen allergy were exposed to a mix of grass pollen allergens or rBet v 1 that had been preincubated with rabbit anti-BM32 IgG antibodies (violet bars), anti-Allergovit IgG (black bars), anti-Alutard IgG (green bars), anti-Phostal IgG (orange bars), anti-Pollinex (blue bars), or IgG from normal rabbits without immunization (before: gray bars; x-axis). Lymphocyte proliferation responses after subtraction of medium background are shown as counts per minute (y-axis). Journal of Allergy and Clinical Immunology 2017 140, 1433-1436.e6DOI: (10.1016/j.jaci.2017.03.048) Copyright © 2017 The Authors Terms and Conditions