Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk Jun Ho Lee, PhD, Jie Wan Kim,

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Presentation transcript:

Curcumin, a constituent of curry, suppresses IgE-mediated allergic response and mast cell activation at the level of Syk Jun Ho Lee, PhD, Jie Wan Kim, MSc, Na Young Ko, MSc, Se Hwan Mun, MSc, Erk Her, PhD, Bo Kyung Kim, PhD, Jeung Whan Han, PhD, Hoi Young Lee, PhD, Michael A. Beaven, PhD, Young Mi Kim, PhD, Wahn Soo Choi, PhD Journal of Allergy and Clinical Immunology Volume 121, Issue 5, Pages 1225-1231 (May 2008) DOI: 10.1016/j.jaci.2007.12.1160 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Curcumin inhibits the mast cell–mediated PCA reaction. A, Each picture shown is representative of 3 independent experiments. B, The means ± SEMs of values from 3 independent experiments, each with 10 mice, are shown. The asterisks indicate a significant difference from controls (no curcumin) at a P value of less than .001. DPH (50 mg/kg) was used as a typical antihistamine control drug. Journal of Allergy and Clinical Immunology 2008 121, 1225-1231DOI: (10.1016/j.jaci.2007.12.1160) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Curcumin reversibly inhibits antigen-induced degranulation in mast cells. A, Release of β-hexosaminidase was measured in antigen-stimulated BMMCs and RBL-2H3 cells. B, Reversibility of curcumin in RBL-2H3 cells. The values indicate the means ± SEMs from 3 independent experiments. Significant differences with controls (no curcumin) are indicated as follows: ∗P < .05 and ∗∗P < .01. PP2 (10 μmol/L) is a general Src-family kinase inhibitor. Journal of Allergy and Clinical Immunology 2008 121, 1225-1231DOI: (10.1016/j.jaci.2007.12.1160) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Curcumin suppresses the expression and secretion of TNF-α and IL-4. A, IgE-primed RBL-2H3 cells were stimulated with antigen or vehicle (NS) for RT-PCR. Densitometric values (means ± SEMs) for the 3 experiments are shown. B, TNF-α and IL-4 concentrations in the culture supernatant were determined by using commercial ELISA kits. Asterisks indicate significant differences from controls as follows: ∗P < .05 and ∗∗P < .01. PP2 (10 μmol/L) is a general Src-family kinase inhibitor. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase. Journal of Allergy and Clinical Immunology 2008 121, 1225-1231DOI: (10.1016/j.jaci.2007.12.1160) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Inhibition of Syk-dependent phosphorylations and Syk kinase activity by curcumin. A, The mast cells were stimulated with DNP-BSA or not (NS) for 7 minutes. The proteins were immunoprecipitated from lysates of RBL-2H3 cells. BMMC lysates were used to detect phosphorylation of Syk and LAT specifically. B, In vitro Syk kinase assay with curcumin. Values indicate the means ± SEMs from 3 independent experiments: ∗∗P < .01. PP2 (10 μmol/L) is a general Src-family kinase inhibitor. Journal of Allergy and Clinical Immunology 2008 121, 1225-1231DOI: (10.1016/j.jaci.2007.12.1160) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 5 Curcumin suppresses phosphorylation of phospholipase Cγ and MAP kinases. A, Immunoblots for phosphorylated and total phospholipase (PL) Cγ1 were prepared from immunoprecipitated PLCγ1 in RBL-2H3 cells. Densitometric data (means ± SEMs) from 3 experiments are shown: ∗P < .05 and ∗∗P < .01. B, Activating phosphorylations of Akt and the indicated MAP kinases were determined by preparation of immunoblots from whole cell lysates. PP2 (10 μmol/L) is a general Src-family kinase inhibitor. Journal of Allergy and Clinical Immunology 2008 121, 1225-1231DOI: (10.1016/j.jaci.2007.12.1160) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions