CsCl centrifugation of DNA over time developed

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Presentation transcript:

CsCl centrifugation of DNA over time developed by Meselson and Stahl

We will talk about this again in a later lecture: But CsCl gradients are not the same thing as Sucrose Gradients or Agarose Gel Electrophoresis.

CsCl centrifugation of DNA over time

N15 is heavier than N14-Can be resolved in CsCl

pulse-chase Experiment: Incubator with N15 containing medium for time, then chase with N14 medium Expt 1 grows Slowly Expt 2 Bacteria Grow Faster Why?

Why would they do 2 different growth rates? Experiment 1 Experiment 2 N14 N15 only N14 N15 only

Fuse Results from Expt 1 and 2 Cell Divisions N14 N15 only

Experiment 1 observations Watson-Crick Model N14 N15 only Does Expt 1 prove hybrid formation?

Critical Experiment: Hybrid Strand Separation And CsCl centrifugation dsDNA N15 ssDNA Critical Experiment: Hybrid Strand Separation And CsCl centrifugation What about N14/N15 hybrid? Looks like control below N15 ssDNA N14 ssDNA

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