Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads  Ester Polak.

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Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads  Ester Polak de Fried, M.D., Flavia Denaday, M.D.  Fertility and Sterility  Volume 94, Issue 1, Pages 351.e15-351.e18 (June 2010) DOI: 10.1016/j.fertnstert.2009.12.037 Copyright © 2010 Terms and Conditions

Figure 1 Case 1: (A) DNA fragmentation analysis done using terminal deoxynucleotide transferase–mediated dUTP nick-end labeling (8); a typical image of fluorescent staining in the sperm head is shown. (B) Micrographs of the day 3 embryos selected for transfer: 5 cell/grade 2 and 6 cell/grade 2–3. Fertility and Sterility 2010 94, 351.e15-351.e18DOI: (10.1016/j.fertnstert.2009.12.037) Copyright © 2010 Terms and Conditions

Figure 2 Case 2: (A) Immunodetection of active caspase-3 (17), showing a typical protein staining in the sperm midpiece. (B) Micrographs of day 3 embryos selected for transfer: 8 cell/grade 1–2 and 7 cell/grade 1. In both cases, embryo classification was done following Lucinda Veek's grading system (26). Fertility and Sterility 2010 94, 351.e15-351.e18DOI: (10.1016/j.fertnstert.2009.12.037) Copyright © 2010 Terms and Conditions

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