Isolation and Identification of Spinach Pigments

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Presentation transcript:

Isolation and Identification of Spinach Pigments Professor Matt And Colleen

Photosynthesis Plants and organisms use for food Requires: Carbon Dioxide Water Sunlight Highly conjugated pigments Metal ions

Pigments Highly conjugated proteins 4 types: Chlorophyll a Chlorophyll b B-carotene Xanthrophylls violaxanthin Lutein Structures provided in lab manual

Chromatography “color graphing” Separation using intermolecular forces Inter = between molecules Capillary action IMF strength due to: Type Shape Size

Rf value Calculation using your measurements Distance moved by a compound, mm Distance moved by the solvent, mm

Polarity Based on: Electronegativity Molecular weight Shape/orientation of molecules

Groups for Absorbency Group 1: 400-430, 600-640