Detection of TERC Amplification in Cervical Epithelial Cells for the Diagnosis of High- Grade Cervical Lesions and Invasive Cancer Jing Jiang, Li-Hui.

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Detection of TERC Amplification in Cervical Epithelial Cells for the Diagnosis of High- Grade Cervical Lesions and Invasive Cancer Jing Jiang, Li-Hui Wei, Ya-Li Li, Rui-Fang Wu, Xing Xie, You-Ji Feng, Guo Zhang, Chao Zhao, Yun Zhao, Zhong Chen The Journal of Molecular Diagnostics Volume 12, Issue 6, Pages 808-817 (November 2010) DOI: 10.2353/jmoldx.2010.100021 Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Representative immunofluorescence images of different cell types stained for TERC and CSP3. A: Normal cells with a CSP3 (green):TERC (red) signal ratio of 2:2. B: A few CIN1 cells with 2:3 or 3:3 signal patterns. C: CIN3 cells exhibiting a 4:4 signal pattern. D: SCC cells with 5:5 and 6:6 signal patterns. Magnification, ×1000. The Journal of Molecular Diagnostics 2010 12, 808-817DOI: (10.2353/jmoldx.2010.100021) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 ROC curves for detection of CIN2+ by cytology, high-risk HPV DNA testing, and TERC amplification analysis. The Journal of Molecular Diagnostics 2010 12, 808-817DOI: (10.2353/jmoldx.2010.100021) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Graphs of the sensitivity (A), specificity (B), and Youden's index values (C) calculated for the methods of cytology, high-risk HPV DNA detection, and TERC amplification analyses for the detection of high-grade cervical lesions and invasive cancer in different age groups. Bars represent 95% confidence intervals. The Journal of Molecular Diagnostics 2010 12, 808-817DOI: (10.2353/jmoldx.2010.100021) Copyright © 2010 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions