Coral Bleaching Experiment By Group 1: L.C. Yao Leo Marcel

Procedure Week 3 Determine Zooxanthelle Density Measure Chlorophyll Concentration Determine Protein Concentration

Coral Bleaching Dispelling of symbiotic zooxanthelle by coral host due to environmental stress Affected by temperature, salinity, amount of UVA radiation, etc

Aiptasia Numbers Mass of Aiptasia = .1396 grams Total Zooxs = 6.4 x 10^6 zoox/mL Zook Density = 92.0 x 10^6 zoox/g wet weight Protein Concent = 1.70 mg/g Chlorophyll ng/10^6 cells = 47.62 ng/10^6 cell

Amount of Chlorophyll In Aiptasia In Xenia Wavelength Absorbance 665nm → .089 647nm → .025 630nm → .045 In Xenia Wavelength Absorbance 665nm → .030 647nm → .015 630nm → .019

Protein Content Aiptasia Xenia Abs(avg) .4895 .743 1.055 1.268 1.480 .1mL of .25 mg/mL BSA + 5mL Bradford .1mL of .50mg/mL BSA + 5mL Bradford .1mL of .75 mg/mL BSA + 5mL Bradford .1mL of 1.00 mg/mL BSA + 5mL Bradford .1mL of 1.25 mg/mL BSA + 5mL Bradford .1 ml of homogenate+ 5mL of Bradford Abs(avg) .4895 .743 1.055 1.268 1.480 2.171 Xenia .1mL of .25 mg/mL BSA + 5mL Bradford .1mL of .50mg/mL BSA + 5mL Bradford .1mL of .75 mg/mL BSA + 5mL Bradford .1mL of 1.00 mg/mL BSA + 5mL Bradford .1mL of 1.25 mg/mL BSA + 5mL Bradford .1 ml of homogenate+ 5mL of Bradford Abs(avg) .385 .641 .900 1.116 1.284 .074

Graph

Calculations Protein: Chlorophyll: Chlorophyll ng/10^6 cells: 2.171 = 1.2895X = 1.695 mg/g Chlorophyll: Ca = (11.85A665)-(1.54A647)-(0.08A630) = .4876 ug/mL Chlorophyll ng/10^6 cells: Ug chl / zoox = (.4876 ug chl / 6.4 x 10^6) = mL mL mL mL 76187.5 ug chl / 1.6cm = 47.62 ng 10^6 cells

Group One’s Coral Bleaching Experiment Coral Experiment Group One’s Coral Bleaching Experiment Hypothesis: Coral will expel zooxs due to an increase in tank temperature Control Tank: Create a tank that remains at a constant temperature, salinity, UVA exposure, etc. Conclusion: Determine if in fact zooxs are expelled because of temperature Each Week: Determine number of zooxs, test cholorphyll and protein, compare with control tank Variable Tank: In this tank, increase the temperature by 3 degrees Celsius and leave at new temp for remaining 3 weeks

THE END!!!