S. Skovbjerg, K. Roos, F. Nowrouzian, M. Lindh, S. E. Holm, I

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Presentation transcript:

High cytokine levels in perforated acute otitis media exudates containing live bacteria S. Skovbjerg, K. Roos, F. Nowrouzian, M. Lindh, S.E. Holm, I. Adlerberth, S. Olofsson, A.E. Wold Clinical Microbiology and Infection Volume 16, Issue 9, Pages 1382-1388 (September 2010) DOI: 10.1111/j.1469-0691.2010.03083.x Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Inflammatory mediators in middle ear effusions with positive bacterial culture, negative culture with positive PCR and negative culture with negative PCR, measured using ELISA. The levels of interleukin (IL)-1β, tumour necrosis factor (TNF), IL-8 and IL-10 were higher in culture-positive middle ear fluids (MEFs) than in culture-negative specimens, irrespective of PCR findings. In contrast, the levels of IL-6 and prostaglandin E2 (PGE2) were equally elevated in effusions with live, dead or undetectable bacteria. *p <0.05, **p <0.01, ***p ≤0.0001. Clinical Microbiology and Infection 2010 16, 1382-1388DOI: (10.1111/j.1469-0691.2010.03083.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 Mononuclear cells from three blood donors were stimulated with clinical acute otitis media (AOM) isolates of UV-inactivated Streptococcus pneumoniae (n = 3), Haemophilus influenzae (n = 3) or Moraxella catarrhalis (n = 1) at optimal concentrations (5 × 107 bacteria/mL), and the levels of inflammatory mediators were compared with the levels measured in middle ear fluids (MEFs) containing these pathogens. Whereas the levels of interleukin (IL)-6 and tumour necrosis factor (TNF) in the MEF samples were similar to those in the supernatants from stimulated leukocytes, the AOM exudates contained more IL-1β and IL-8, but less IL-10, than was obtained in vitro. **p <0.001, ***p <0.0001. Clinical Microbiology and Infection 2010 16, 1382-1388DOI: (10.1111/j.1469-0691.2010.03083.x) Copyright © 2010 European Society of Clinical Infectious Diseases Terms and Conditions