Cystic fibrosis transmembrane conductance regulator (CFTR) regulates the production of osteoprotegerin (OPG) and prostaglandin (PG) E2 in human bone

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Cystic fibrosis transmembrane conductance regulator (CFTR) regulates the production of osteoprotegerin (OPG) and prostaglandin (PG) E2 in human bone Louis Le Heron, Christine Guillaume, Frédéric Velard, Julien Braux, Lhousseine Touqui, Sandra Moriceau, Isabelle Sermet-Gaudelus, Dominique Laurent-Maquin, Jacky Jacquot Journal of Cystic Fibrosis Volume 9, Issue 1, Pages 69-72 (January 2010) DOI: 10.1016/j.jcf.2009.11.005 Copyright © 2009 European Cystic Fibrosis Society. Terms and Conditions

Fig. 1 Panel A: CFTR mRNA expression of human osteoblasts in primary culture (lane 2) compared to positive control human bronchial cells (16HBE14-cell line, lane 1) analyzed by RT-PCR; PCR products were separated by 1% agarose gel electrophoresis and visualized with ethidium bromide with an ImageMaster® VDS (Pharmacia Biotech). Panel B: CFTR protein immunofluorescence in normal human osteoblasts (passage 3, 7week-culture). Journal of Cystic Fibrosis 2010 9, 69-72DOI: (10.1016/j.jcf.2009.11.005) Copyright © 2009 European Cystic Fibrosis Society. Terms and Conditions

Fig. 2 Panels A and B: Osteoprotegerin (OPG) and prostaglandin E2 (PGE2) secretion (pg/ml/106 cells) by cultured human osteoblasts and maintained for a 5-h period in the absence (−) and presence (+) of TNF-α (20ng/ml) following a 72-h pretreatment of 10μM of CFTR-inh172, respectively. ** Significantly different (p<0.05) from control (DMSO). Results are the mean and S.E. of five independent experiments (realized in triplicate). Journal of Cystic Fibrosis 2010 9, 69-72DOI: (10.1016/j.jcf.2009.11.005) Copyright © 2009 European Cystic Fibrosis Society. Terms and Conditions