P. -J. Francin, A. Abot, C. Guillaume, D. Moulin, A. Bianchi, P

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Association between adiponectin and cartilage degradation in human osteoarthritis P.-J. Francin, A. Abot, C. Guillaume, D. Moulin, A. Bianchi, P. Gegout-Pottie, J.-Y. Jouzeau, D. Mainard, N. Presle Osteoarthritis and Cartilage Volume 22, Issue 3, Pages 519-526 (March 2014) DOI: 10.1016/j.joca.2014.01.002 Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Association between the synthesis of adiponectin and cartilage degradation. A. Production of adiponectin in representative sections from cartilage biopsies obtained in healthy individuals and OA patients. The control staining was performed without primary antibody. Positive cells are coloured brown and nuclei are counterstained with haematoxylin (original magnification ×40). B. BMI-dependent association between the production of adiponectin and the grade of cartilage destruction. Full-depth cartilage biopsies (n = 84) obtained from 11 OA patients were cultured for 48 h and then histologically graded according to the Mankin score. Adiponectin production was determined in duplicate for each cartilage sample. Horizontal lines indicate means with 95% confidence intervals and each dot represents one cartilage specimen. Osteoarthritis and Cartilage 2014 22, 519-526DOI: (10.1016/j.joca.2014.01.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Correlations between the mRNA expression of adiponectin and that of the microsomal prostaglandin E synthase (mPGES), the matrix metalloprotease-13 (MMP-13), the aggrecan, Sox9 and collagen type 2 in cartilage from OA patients (n = 11). Osteoarthritis and Cartilage 2014 22, 519-526DOI: (10.1016/j.joca.2014.01.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Correlations between the mRNA expression of adiponectin receptor 1 (AdipoR1) and that of the microsomal prostaglandin E synthase (mPGES), the matrix metalloprotease-13 (MMP-13), the aggrecan, Sox9 and collagen type 2 in cartilage from OA patients (n = 11). Osteoarthritis and Cartilage 2014 22, 519-526DOI: (10.1016/j.joca.2014.01.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Dose-dependent effect of the full-length or the globular form of adiponectin on the activity of the matrix metalloprotease-13 (MMP-13) and the prostaglandin E2 (PGE2) production in chondrocytes collected from six OA patients. Experiment was carried out in triplicate for each patient. Horizontal lines indicate means with 95% confidence intervals and each dot represents the data found with the chondrocytes from one patient. * = P < 0.05 between adiponectin-treated and unstimulated chondrocytes with n = 6. Osteoarthritis and Cartilage 2014 22, 519-526DOI: (10.1016/j.joca.2014.01.002) Copyright © 2014 Osteoarthritis Research Society International Terms and Conditions