Lineage-Restricted Expression of Protein Kinase C Isoforms in Hematopoiesis by Alessandra Bassini, Giorgio Zauli, Giovanni Migliaccio, Anna Rita Migliaccio,

Slides:

Advertisements

Similar presentations

Increase in Elastin Gene Expression and Protein Synthesis in Arterial Smooth Muscle Cells Derived From Patients with Moyamoya Disease by Mari Yamamoto,

Advertisements

Functional role of alternatively spliced deoxycytidine kinase in sensitivity to cytarabine of acute myeloid leukemic cells by Marjan J. T. Veuger, Mirjam.

Involvement of CD147 on multidrug resistance through the regulation of P-glycoprotein expression in K562/ADR leukemic cell line Aoranit Somno, Songyot.

Arterioscler Thromb Vasc Biol

Coexistence of multiple mechanisms of PT523 resistance in human leukemia cells harboring 3 reduced folate carrier alleles: transcriptional silencing, inactivating.

A study of the coregulation and tissue specificity of XGand MIC2 gene expression in eukaryotic cells by Claude Fouchet, Pierre Gane, Martine Huet, Marc.

Htr-9 stimulates ICAM-1 gene expression in human ASM cells.

by Karen Reue, Robert D. Cohen, and Michael C. Schotz

REDK, a novel human regulatory erythroid kinase

by V.M. Sellers, T.A. Dailey, and H.A. Dailey

In vivo administration of vascular endothelial growth factor (VEGF) and its antagonist, soluble neuropilin-1, predicts a role of VEGF in the progression.

The Expression of Bcl-x Is Downregulated During Differentiation of Human Hematopoietic Progenitor Cells Along the Granulocyte But Not the Monocyte/Macrophage.

Characterization of the Human Platelet/Endothelial Cell Adhesion Molecule-1 Promoter: Identification of a GATA-2 Binding Element Required for Optimal Transcriptional.

by Jurgen Seppen, Simon C. Barry, Brandon Harder, and William R. A

Antiangiogenic antithrombin down-regulates the expression of the proangiogenic heparan sulfate proteoglycan, perlecan, in endothelial cells by Weiqing.

by Madhu Gupta, Paul T. Mungai, and Eugene Goldwasser

Hypomethylation Status of CpG Sites at the Promoter Region and Overexpression of the Human MDR1 Gene in Acute Myeloid Leukemias by Masaharu Nakayama, Morimasa.

Upregulation of Cortactin Expression During the Maturation of Megakaryocytes by Xi Zhan, Christian C. Haudenschild, Yangson Ni, Elizabeth Smith, and Cai.

Activation of JAK2 in Human Vascular Endothelial Cells by Granulocyte-Macrophage Colony-Stimulating Factor by Raffaella Soldi, Luca Primo, Maria Felice.

Cyclophosphamide induces type I interferon and augments the number of CD44hi T lymphocytes in mice: implications for strategies of chemoimmunotherapy of.

STAT3-mediated constitutive expression of SOCS-3 in cutaneous T-cell lymphoma by Christine Brender, Mette Nielsen, Keld Kaltoft, Gitte Mikkelsen, Qian.

Molecular characterization and expression of a novel human leukocyte cell-surface marker homologous to mouse Ly-9 by Miguel Angel de la Fuente, Victoria.

Molecular characterization of in-frame and out-of-frame alternative splicings in coagulation factor XI pre-mRNA by Rosanna Asselta, Valeria Rimoldi, Ilaria.

FOG-1 represses GATA-1-dependent FcϵRI β-chain transcription: transcriptional mechanism of mast-cell-specific gene expression in mice by Keiko Maeda, Chiharu.

CD74 induces TAp63 expression leading to B-cell survival

Regulation of monocyte migration by amphoterin (HMGB1)‏

by Takashi Kasukabe, Junko Okabe-Kado, and Yoshio Honma

Severe hemochromatosis in a Portuguese family associated with a new mutation in the 5′-UTR of the HAMP gene by Thomas Matthes, Patricia Aguilar-Martinez,

Exposure to fluid shear stress modulates the ability of endothelial cells to recruit neutrophils in response to tumor necrosis factor-α: a basis for local.

Volume 60, Issue 2, Pages (August 2001)

Lawrence J. Macala, John P. Hayslett, Joan I. Smallwood

Characterization and functional analysis of laminin isoforms in human bone marrow by Ulrich Siler, Martina Seiffert, Sabine Puch, Allan Richards, Beverly.

by Hui Xu, Mark S. Williams, and Lisa M. Spain

Volume 114, Issue 5, Pages (May 1998)

by Xiaowu Zhang, and Ruibao Ren

Circulating forms of intercellular adhesion molecule (ICAM)-1 in mice lacking membranous ICAM-1 by Natasja K. van den Engel, Edmund Heidenthal, Antje Vinke,

Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator by Yan Jiao, John Wilkinson, Xiumin Di, Wei Wang,

D3: A Gene Induced During Myeloid Cell Differentiation of Linlo c-Kit+ Sca-1+ Progenitor Cells by Sarah R. Weiler, John M. Gooya, Mariaestela Ortiz, Schickwann.

Inhibition of glycogen synthase kinase-3 activity leads to epigenetic silencing of nuclear factor κB target genes and induction of apoptosis in chronic.

by Guang Yang, Shu-Ching Huang, Jane Y. Wu, and Edward J. Benz

Vascular endothelial growth factor stimulates protein kinase CβII expression in chronic lymphocytic leukemia cells by Simon T. Abrams, Benjamin R. B. Brown,

Gene expression and immunologic consequence of SPAN-Xb in myeloma and other hematologic malignancies by Zhiqing Wang, Yana Zhang, Haichao Liu, Emanuela.

The (4;11)(q21;p15) Translocation Fuses the NUP98 andRAP1GDS1 Genes and Is Recurrent in T-Cell Acute Lymphocytic Leukemia by Damian J. Hussey, Mario Nicola,

by Yuka Nagata, Eisuke Nishida, and Kazuo Todokoro

Yang et al. SALL4 is a key regulator of survival and apoptosis in human leukemic cells. Blood. 2008;112(3):805–813. Blood Volume 114(14): October.

Detection of Protein Tyrosine-Kinase (PTK) Gene Expression Pattern in Normal and Malignant T Lymphocytes by Combined PTK-Specific Polymerase Chain Reaction.

by Kwang-Hyun Baek, Michelle A

CTLA-4 is not restricted to the lymphoid cell lineage and can function as a target molecule for apoptosis induction of leukemic cells by Maria Pia Pistillo,

Three differentially expressed survivin cDNA variants encode proteins with distinct antiapoptotic functions by Edward M. Conway, Saskia Pollefeyt, Jan.

Bone morphogenetic protein-2 induces apoptosis in human myeloma cells with modulation of STAT3 by Chiharu Kawamura, Masahiro Kizaki, Kenji Yamato, Hideo.

Supplementary Figure 1 RT-PCR BclxL mismatch sc35 Dox BclxS

Expression of inter-α-trypsin inhibitor and tumor necrosis factor-stimulated gene 6 in renal proximal tubular epithelial cells Ulf Janssen, Gareth Thomas,

Volume 54, Issue 2, Pages (August 1998)

Soluble CD86 Is a Costimulatory Molecule for Human T Lymphocytes

Volume 17, Issue 3, Pages (September 2002)

Volume 58, Issue 4, Pages (October 2000)

Volume 67, Issue 1, Pages (January 2005)

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Volume 4, Issue 6, Pages (December 2001)

Volume 118, Issue 5, Pages (May 2000)

Expression and activation of caspase-3/CPP32 in CD34+ cord blood cells is linked to apoptosis after growth factor withdrawal Li-Sheng Wang, Hong-Jun.

Volume 56, Issue 5, Pages (November 1999)

Immunoblots of PLCβ isoforms in wild-type and PLCβ2/β3-null platelets.

Expression of Opsin Molecule in Cultured Murine Melanocyte

Lin Mu, Ph. D. , Wei Zheng, Ph. D. , M. D. , Liang Wang, Ph. D

BORIS mRNA expression in DNMT deficient HCT116 cell lines.

RT-PCR Gel Blot Analyses of Cucurbita PP1 and PP2 mRNAs from Intergeneric Grafts of Cucumis sativus Scions on Cucurbita maxima or Cucurbita ficifolia Stocks.RT-PCR.

CHL-induced apoptosis in HCT116 cells involves the activation of procaspase-8 and procaspase-6, with cleavage of nuclear lamins. CHL-induced apoptosis.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

Matrix metalloproteinase and tissue inhibitor of matrix metalloproteinase expression in human preimplantation embryos Hongbo Wang, M.D., Yan Wen, M.D.,

Presentation transcript:

Lineage-Restricted Expression of Protein Kinase C Isoforms in Hematopoiesis by Alessandra Bassini, Giorgio Zauli, Giovanni Migliaccio, Anna Rita Migliaccio, Massimiliano Pascuccio, Sabina Pierpaoli, Lia Guidotti, Silvano Capitani, and Marco Vitale Blood Volume 93(4):1178-1188 February 15, 1999 ©1999 by American Society of Hematology

Western blot analysis of PKC-, βΙ , δ, ɛ, η, and ζ isoforms in homogenates obtained from freshly isolated CD34+ cells. Western blot analysis of PKC-, βΙ , δ, ɛ, η, and ζ isoforms in homogenates obtained from freshly isolated CD34+ cells. Isoform-specific antibodies were added to the membranes in the absence (left of each couple lanes) or presence of the corresponding immunizing peptide (right of each couple of lanes). A representative of three separate experiments is reported. Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Assay of PKC catalytic activity in homogenates obtained from various 32D subclones. Assay of PKC catalytic activity in homogenates obtained from various 32D subclones. Data are reported as the means ± SD of three separate experiments performed in duplicate. Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology

RT-PCR analysis of the expression of PKC-ɛ and - mRNA in the subclones of the 32D cell lines. RT-PCR analysis of the expression of PKC-ɛ and - mRNA in the subclones of the 32D cell lines. The ethidium bromide staining of products amplified by 30 cycles of PCR with primers specific for the PKC-ɛ and - or for murine actin and separated by electrophoresis on agarose gel are presented. Comparable amounts of products of the expected molecular weights were amplified not only from cDNA obtained from human cord blood (last lane) and mouse bone marrow (first lane) mononuclear cells, as controls, but also from cDNA obtained from all subclones of the 32D cell lines. The specificity of the PKC-ɛ band amplified from the 32D Epo cell line was proved by direct sequencing of the band eluted from the gel. Alessandra Bassini et al. Blood 1999;93:1178-1188 ©1999 by American Society of Hematology