Figure 1: Serum LDH levels elevated significantly in KD and febrile non-KD patients. LDH levels were respectively measured in KD, compared with FC and.

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Figure 1: Serum LDH levels elevated significantly in KD and febrile non-KD patients. LDH levels were respectively measured in KD, compared with FC and HC. Compared with HC, sera LDH levels were significantly increase in KD (p=0.0352) and febrile controls (p=0.0005), suggesting LDH not a specific marker for KD diagnosis. FC: fertile non-KD control.

Figure 2: Plasmid construction, protein expression and purification of LDHA. LDHA was over expressed according to standard procedures. LDHA gene was amplified by PCR and then analyzed by agarose gel electrophoresis in Panel A. Panel B showed a diagrammed process, including digestion, plasmid construction and transformation. Whole cell lysate protein from four recombinant E. Coli were respectively electrophoresed (Lane 2-5 in Panel C), compared with blank E. Coli control (Lane 1), blank plasmid control (Lane 7) and IPTG control (Lane 6). After ultrasonication, the majority of LDHA protein was detected in the precipitation (Lane 2 in Panel D), barely in the supernatant (Lane 1). Panel E showed purification of LDHA, followed by MS identification in Panel F.

Figure 3: ELISA analysis of serum anti-LDHA IgA, IgG and IgM antibody levels. Panel A showed a significant elevation of serum anti-LDHA IgA levels in KD patients, compared with FC (p=0.035). For anti-LDHA IgG analysis in Panel B, an increase was also detected in KD compared with HC (p=0.001). While a decline was observed in serum anti-LDHA IgM levels in Panel C, compared with FC (p=0.020) and HC (p=0.038). Panel D-F respectively showed correlation analysis between serum LDH levels and anti-LDHA IgA, IgG and IgM levels, and the results proved no obvious linear relationship.