Creation of Chimeric Hearts: A Tool for Testing the “Passenger Leukocyte” Hypothesis Simon D Eiref, Wei Zhang, Sicco H Popma, Lisa J Shah, Jonni S Moore,

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Creation of Chimeric Hearts: A Tool for Testing the “Passenger Leukocyte” Hypothesis Simon D Eiref, Wei Zhang, Sicco H Popma, Lisa J Shah, Jonni S Moore, Bruce R Rosengard The Annals of Thoracic Surgery Volume 64, Issue 3, Pages 628-633 (September 1997) DOI: 10.1016/S0003-4975(97)00617-6

Fig. 1 Kaplan-Meier survival curves for B10 bone marrow transplantation (BMT) recipients. B10 mice were lethally irradiated (10 Gy) and were given no marrow (dashed line), autologous B10 marrow (dotted line), or allogeneic C3H marrow (solid line). Survival of allogeneic C3H → B10 and autologous B10 → B10 BMTs were not significantly different (p = 0.406, Breslow). Survival of both autologous and allogeneic BMTs was significantly improved as compared with irradiation controls (p < 0.0004, Breslow). The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)

Fig. 2 Kaplan-Meier survival curves for BALB/c BMT recipients. BALB/c mice were lethally irradiated (10 Gy) and were given no marrow (dashed line), autologous BALB/c marrow (dotted line), or allogeneic CBA marrow (solid line). Survival of allogeneic CBA → BALB/c BMTs was significantly lower (p = 0.008, Breslow) than autologous BALB/c → BALB/c BMTs. Survival of both autologous and allogeneic BMTs was significantly improved as compared with irradiation controls (p < 0.0002, Breslow). The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)

Fig. 3 Flow cytometric analysis of an antigen-presenting cell (APC)-enriched fraction isolated from a chimeric B10 heart (C3H → B10 BMT): (a) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 25-9-17 (solid line), an anti-I-Ab/d (recipient B10 class II major histocompatibility complex [MHC] antigen) reagent. (b) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 11-5.2 (solid line), an anti-I-Ak (donor C3H class II MHC antigen) reagent. The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)

Fig. 4 Flow cytometric analysis of an APC-enriched fraction isolated from a chimeric BALB/c heart (CBA → BALB/c BMT): (a) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 25-9-17 (solid line), an anti-I-Ab/d (recipient BALB/c class II MHC antigen) reagent. (b) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 11-5.2 (solid line), an anti-I-Ak (donor CBA class II MHC antigen) reagent. The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)

Fig. 5 Flow cytometric analysis of an APC-enriched fraction isolated from a chimeric B10 spleen (C3H → B10 BMT): (a) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 25-9-17 (solid line), an anti-I-Ab/d (recipient B10 class II MHC antigen) reagent. (b) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 11-5.2 (solid line), an anti-I-Ak (donor C3H class II MHC antigen) reagent. The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)

Fig. 6 Flow cytometric analysis of an APC-enriched fraction isolated from a chimeric BALB/c spleen (CBA → BALB/c BMT): (a) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 25-9-17 (solid line), an anti-I-Ab/d (recipient BALB/c class II MHC antigen) reagent. (b) Staining with fluoresceinated monoclonal antibodies G155-178 (dashed line), an immunoglobulin G2a isotype control of irrelevant specificity, and 11-5.2 (solid line), an anti-I-Ak (donor CBA class II MHC antigen) reagent. The Annals of Thoracic Surgery 1997 64, 628-633DOI: (10.1016/S0003-4975(97)00617-6)