Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana,

Slides:

Advertisements

Similar presentations

Hepatitis B Virus. Hepatitis B - Clinical Features Incubation period:Average days Range days Clinical illness (jaundice):

Advertisements

Virus discovery-454 sequencing

WRLFMD Identification of a Ninth Foot-and-Mouth Disease Virus Type O Topotype and Evidence for a Recombination Event in its Evolution Nick J. Knowles,

1/03/09 De 89 à 98. 1/03/09 De 89 à 98 1/03/09 De 89 à 98.

Transcriptomics Breakout. Topics Discussed Transcriptomics Applications and Challenges For Each Systems Biology Project –Host and Pathogen Bacteria Viruses.

High Throughput Sequencing

Laboratory Diagnosis of Virus Infections

Next Generation Sequencing and its data analysis challenges Background Alignment and Assembly Applications Genome Epigenome Transcriptome.

B19 inactivation by heat treatment with epithelial cell lines Yoshiaki Okada Kiyoko Umemori Kazunari Yamaguchi National Institute of Infectious Disease.

Molecular biology and evolution in genomics era Li Jun and Frederick C.C. Leung 5N01, School of Biological Science, The University.

***. The Air Car 2 nd Quarter Independent Study 1934.

HW2: exome sequencing and complex disease Jacquemin Jonathan de Bournonville Sébastien.

LABORATORY VALIDATION OF TWO REAL TIME RT-PCR METHODS FOR FOOT-AND-MOUTH DISEASE VIRUS WITH AN INCREASED DIAGNOSTIC SPECIFICITY Vandenbussche Frank, Lefebvre.

Risk assessment: The Safety of Blood Products presented by Dr. Thomas R. Kreil Baxter BioScience on behalf of the PPTA Pathogen Safety Steering Committee.

Algebra 1 cc Function 4 Evaluate and write arithmetic sequences Recall: A function is relation in which each element of the domain is paired with exactly.

Reliable Identification of Genomic Variants from RNA-seq Data Robert Piskol, Gokul Ramaswami, Jin Billy Li PRESENTED BY GAYATHRI RAJAN VINEELA GANGALAPUDI.

Genomic analysis of three African strains of Bacillus anthracis demonstrates that they are part of the clonal expansion of an exclusively pathogenic bacterium.

。 33 投资环境 3 开阔视野提升竞争力。 3 嘉峪关市概况。 3 。 3 嘉峪关是一座新兴的工业旅游城市，因关得名，因企设市，是长城文化与丝路文化交汇点，是全国唯一一座以长城关隘命名的城市。嘉峪关关城位于祁连山、黑山之间。 1965 年建市，下辖雄关区、镜铁区、长城区，全市总面积 2935.

ΜΕΤΑΣΥΛΛΕΚΤΙΚΗ ΦΥΣΙΟΛΟΓΙΑ ΕΡΓΑΣΤΗΡΙΟ 3. Μετασυλλεκτική Εργ3-Λιοσάτου Γ.2 ΒΙΟΛΟΓΙΚΟΙ ΠΑΡΑΓΟΝΤΕΣ ΠΟΥ ΕΠΗΡΕΑΖΟΥΝ ΤΗ ΦΘΟΡΑ ΤΩΝ ΟΠΩΡΟΚΗΠΕΥΤΙΚΩΝ Αναπνοή Η λειτουργία.

Interpreting exomes and genomes: a beginner’s guide

Advances in Genetics Key Concepts

Development and Evaluation of SYBR Green-Based Real-time PCR for Detection of Echinococcus granulosus Hydatid cysts Recovered from Humans and Livestock.

Foot and Mouth Disease Ministry Of Agriculture, Kingdom of Saudi Arabia

Arithmetic and Geometric Means

Use of next generation Sequencing (NGS) at a National Reference Laboratory, Denmark 2015/16 Authors: Thea Kølsen Fischer, Ramona Trebbien, Jannik Fonager,

A. Latsuzbaia¹, J. Tapp¹, C. Ragimbeau¹, M. Fischer, S. Weyers², M

P. Code (A ) Material and Methods Results Conclusion

Efficacy of a Foot-and-Mouth Disease inactivated vaccine (AFTOVAXPUR DOE), administered at a 1 mL dose to sheep Claude Hamers.

Rapid and sensitive diagnosis of Toxoplasma gondii infections by PCR

Figure 1 The genomic nephrology workflow: genetic diagnosis and clinical application Figure 1 |The genomic nephrology workflow: genetic diagnosis and clinical.

Outbreak of brainstem encephalitis associated with enterovirus-A71 in Catalonia, Spain (2016): a clinical observational study in a children's reference.

Development of One step Multiplex RT-PCR for differentiation of FMDV serotypes A, O and SAT2 in Egypt Amir A. Shehata.

اثرات گرمايش جهاني تغييرات آب و هوا، تأثيرات عميق و شديدي بر بسياري از عوامل اساسي موثر بر سلامت از جمله : آب، غذا، هوا و محيط زيست دارد كه اين مورد خود.

Simultaneous single-tube PCR assay for the detection of Neisseria meningitidis, Haemophilus influenzae type b and Streptococcus pneumoniae G. Tzanakaki,

A novel strategy based on genomics and specific PCR reveals how a multidrug-resistant Mycobacterium tuberculosis strain became prevalent in Equatorial.

The Human Genome Project and beyond

Department of Biotechnology University of Malakand, Pakistan

ICAR-Directorate of Foot-and-mouth disease, Mukteswar, India

B. Edvinsson, M. Lappalainen, B. Evengård

Characterization of FMDV isolates from Sudan collected from outbreaks

Large-scale testing of women in Copenhagen has not reduced the prevalence of Chlamydia trachomatis infections H. Westh, H.J. Kolmos Clinical Microbiology.

COMPETITIVE LUMINEX IMMUNOASSAYS FOR THE DETECTION OF ANTIBODIES TO FOOT-AND-MOUTH DISEASE AND VESICULAR STOMATITIS VIRUSES IN MULTIPLE SUSCEPTIBLE HOSTS.

FMD Reference Laboratory

Barsha Thapa, Jemma Wadsworth, Nick J. Knowles and Donald P. King

Comparison of microscopy, real-time PCR and a rapid immunoassay for the detection of Giardia lamblia in human stool specimens T. Schuurman, P. Lankamp,

Evaluation of viral co-infections in hospitalized and non-hospitalized children with respiratory infections using microarrays S. Kouni, P. Karakitsos,

4n + 2 1st term = 4 × = 6 2nd term = 4 × = 10 3rd term

Metagenomics and development of the gut microbiota in infants

Advances in Genetics Key Concepts

Nucleotide sequences encoding part of the VP1 capsid protein of FMDVs derived from infected cattle in phase I and phase II of the study. Nucleotide sequences.

Immune responses to foot-and-mouth disease virus in guinea pigs after vaccination with canine adenovirus vector S.A. Lacour, A. De Vleeschauwer, L. Bakkali-Kassimi,

Visual detection of IS6110 of Mycobacterium tuberculosis in sputum samples using a test based on colloidal gold and latex beads P. Upadhyay, M. Hanif,

Evaluation of a new 5'-nuclease real-time PCR assay targeting the Toxoplasma gondii AF genomic repeat J. Menotti, Y. J-F. Garin, P. Thulliez, M-C.

TAILED PRIMERS ENHANCE REAL-TIME RT-PCR DETECTION OF FOOT AND MOUTH DISEASE VIRUS F. Vandenbussche, E. Mathijs, D. Lefebvre, K. De Clercq and S. Van Borm.

The SOP generates high-quality full-genome sequence data across multiple ssRNA+ virus families. The SOP generates high-quality full-genome sequence data.

A retrospective study into the prevalence of herpes simplex virus 1&2 in female patients tested for Chlamydia trachomatis and/or Neisseria gonorrhoeae.

Toscana virus meningitis case in Switzerland: an example of the ezVIR bioinformatics pipeline utility for the identification of emerging viruses S. Cordey,

European quality clearance of new microbiological diagnostics

Detection of, and frequent co-infection with, human bocavirus in faecal specimens from children in Wuhan, China Y. Huang, P. Mao, H. Wang Clinical Microbiology.

Performing the SOP with both HRV-16 and FMDV to identify where loss of genomic RNA infectivity occurs. Performing the SOP with both HRV-16 and FMDV to.

Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective.

F. M. Moesker, J. J. A. van Kampen, A. A. van der Eijk, A. M. C

Multiplex DNA amplification and barcoding in a single reaction for 454 Roche sequencing: A comprehensive study on the control region of the mitochondrial.

C.2.10 Sample Questions.

Molecular epidemiology of enterovirus 71, coxsackievirus A16 and A6 associated with hand, foot and mouth disease in Spain M. Cabrerizo, D. Tarragó, C.

C.2.8 Sample Questions.

C.2.8 Sample Questions.

FMDV infection dynamics in cattle in phase II of the study.

Biotechnology Mader 19.4.

Epidemiological parameters from transmission experiments: new methods for old data Simon Gubbins, David Schley & Ben Hu Transmission Biology Group The.

Presentation transcript:

Complete Genome Sequences of Three African Foot-and-Mouth Disease Viruses, Directly from Clinical Samples S Van Borm, T Rosseel, A Haegeman, M E Fana, L Seoke, J Hyera, G Matlho, F Vandenbussche, K De Clercq

The Study Can unbiased RNASeq produce complete FMDV genome sequences directly from clinical samples? 3 strong positive epithelial samples (CT range 14,63 – 16,18) from Zambia and Namibia

Results Unbiased RNASeq produces complete genomes with high average coverage in 2/3 samples limited gap closure (Sanger sequencing) needed in 3rd sample due to low coverage (limited % of FMDV reads). Potential for unbiased detection of co-infections If only targeting FMDV, targeted approaches may produce better coverage. e.g. Logan G, Freimanis GL, King DJ, Valdazo-Gonzalez B, Bachanek-Bankowska K, Sanderson ND, Knowles NJ, King DP, Cottam EM. (2014) BMC Genomics 15:828

Conclusion These data demonstrate the feasibility of direct sequencing of complete FMDV coding sequences from samples from symptomatic animals The complete genome sequences of O/ZAM14/2010, SAT1/NAM01/2010 and SAT2/ZAM18/2009 were obtained