The costimulatory receptor B7-1 is not induced in injured podocytes

Slides:



Advertisements
Similar presentations
Volume 72, Issue 2, Pages (July 2007)
Advertisements

Protein Tyrosine Phosphatase 1B Inhibition Protects against Podocyte Injury and Proteinuria  Takanori Kumagai, Cindy Baldwin, Lamine Aoudjit, Lisa Nezvitsky,
Takehiko Wada, Jeffrey W. Pippin, Yoshio Terada, Stuart J. Shankland 
MDM2 (murine double minute-2) links inflammation and tubular cell healing during acute kidney injury in mice  Shrikant R. Mulay, Dana Thomasova, Mi Ryu,
Volume 78, Issue 12, Pages (December 2010)
Volume 86, Issue 4, Pages (October 2014)
Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model  Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.
F. Guan, G. Villegas, J. Teichman, P. Mundel, A. Tufro 
Volume 82, Issue 7, Pages (October 2012)
Volume 94, Issue 3, Pages (September 2018)
Volume 82, Issue 5, Pages (September 2012)
Volume 18, Issue 3, Pages (September 2013)
Mesenchymal Stem Cells Ameliorate Podocyte Injury and Proteinuria in a Type 1 Diabetic Nephropathy Rat Model  Shuai Wang, Yi Li, Jinghong Zhao, Jingbo.
Canonical Wnt/β-catenin signaling mediates transforming growth factor-β1-driven podocyte injury and proteinuria  Dan Wang, Chunsun Dai, Yingjian Li, Youhua.
Inhibition of ILK activity does not affect normal kidney structure and nephrin/ILK interaction in vivo. Inhibition of ILK activity does not affect normal.
Jacob Green, Ruth Goldberg, Gila Maor  Kidney International 
Volume 80, Issue 12, Pages (December 2011)
Volume 88, Issue 4, Pages (October 2015)
Volume 85, Issue 2, Pages (January 2014)
Volume 84, Issue 5, Pages (November 2013)
Regulation and Function of the Caspase-1 in an Inflammatory Microenvironment  Dai-Jen Lee, Fei Du, Shih-Wei Chen, Manando Nakasaki, Isha Rana, Vincent.
Volume 78, Issue 12, Pages (December 2010)
Kathryn J. Moore, Takashi Wada, Susannah D. Barbee, Vicki Rubin Kelley 
Volume 72, Issue 4, Pages (August 2007)
Volume 68, Issue 6, Pages (December 2005)
Volume 83, Issue 5, Pages (May 2013)
Proteomic analysis of the slit diaphragm complex: CLIC5 is a protein critical for podocyte morphology and function  Brian A. Pierchala, Maura R. Muñoz,
Volume 84, Issue 5, Pages (November 2013)
Volume 87, Issue 2, Pages (February 2015)
Volume 25, Issue 11, Pages (November 2017)
Volume 85, Issue 6, Pages (June 2014)
Volume 75, Issue 11, Pages (June 2009)
Volume 56, Issue 5, Pages (November 1999)
Volume 66, Issue 4, Pages (October 2004)
Mesenchymal, but not hematopoietic, stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction in mice  Keiichi Fukuda,
Volume 77, Issue 11, Pages (June 2010)
Volume 82, Issue 9, Pages (November 2012)
Volume 62, Issue 3, Pages (September 2002)
Volume 27, Issue 1, Pages (July 2007)
Volume 87, Issue 2, Pages (February 2015)
Toll-Like Receptor 4 Has an Essential Role in Early Skin Wound Healing
MDM2 (murine double minute-2) links inflammation and tubular cell healing during acute kidney injury in mice  Shrikant R. Mulay, Dana Thomasova, Mi Ryu,
Volume 93, Issue 1, (January 2018)
Role of meprin A in renal tubular epithelial cell injury
Volume 73, Issue 4, Pages (February 2008)
Volume 10, Issue 2, Pages (February 2018)
Role of the retinoic acid receptor-α in HIV-associated nephropathy
Volume 82, Issue 7, Pages (October 2012)
S100A15, an Antimicrobial Protein of the Skin: Regulation by E
Renoprotective role of the vitamin D receptor in diabetic nephropathy
Volume 21, Issue 4, Pages (April 2013)
P. Harding, L. Balasubramanian, J. Swegan, A. Stevens, W.F. Glass 
Prevention of mesangial sclerosis by bone marrow transplantation
Volume 70, Issue 10, Pages (November 2006)
Interleukin-17A blockade reduces albuminuria and kidney injury in an accelerated model of diabetic nephropathy  Carolina Lavoz, Yenniffer Sánchez Matus,
Volume 80, Issue 5, Pages (September 2011)
Syed M. Meeran, Thejass Punathil, Santosh K. Katiyar 
Volume 57, Issue 1, Pages (January 2000)
Volume 89, Issue 4, Pages (April 2016)
Volume 72, Issue 2, Pages (July 2007)
Volume 69, Issue 11, Pages (June 2006)
Volume 85, Issue 1, Pages (January 2014)
IL-17A Upregulates Keratin 17 Expression in Keratinocytes through STAT1- and STAT3- Dependent Mechanisms  Xiaowei Shi, Liang Jin, Erle Dang, Ting Chang,
R. Tapia, F. Guan, I. Gershin, J. Teichman, G. Villegas, A. Tufro 
Volume 72, Issue 2, Pages (July 2007)
Volume 68, Issue 1, Pages (July 2005)
Volume 24, Issue 1, Pages (January 2006)
Kathryn J. Moore, Takashi Wada, Susannah D. Barbee, Vicki Rubin Kelley 
TGF-β1 down-regulates induced expression of both class II MHC and B7-1 on primary murine renal tubular epithelial cells  Nazifa Banu, Catherine M. Meyers 
Microfluidic platform for cell phenotype and gene function analysis
Presentation transcript:

The costimulatory receptor B7-1 is not induced in injured podocytes Emilie Baye, Morgan Gallazzini, Marianne Delville, Christophe Legendre, Fabiola Terzi, Guillaume Canaud  Kidney International  Volume 90, Issue 5, Pages 1037-1044 (November 2016) DOI: 10.1016/j.kint.2016.06.022 Copyright © 2016 International Society of Nephrology Terms and Conditions

Figure 1 B7-1 is not induced in primary cultured podocytes after injury. (a) Coimmunostaining for nephrin (green), nestin (red), and podocin (purple) in mouse podocytes isolated from primary culture. (b) B7-1 mRNA is barely detectable in podocytes at baseline and is not induced after lipopolysaccharide (LPS) stimulation. IL-6 and IL-1β mRNA confirmed Toll-like receptor 4 engagement after LPS treatment. (c) Western blot of B7-1 (SC-9091 from Santa Cruz Biotechnology Inc., Heidelberg, Germany) in primary cultures of mouse podocytes exposed to either vehicle or 50.0 μg/ml LPS for 24 hours. C+: positive control, HeLa cells transfected with a vector containing mouse B7-1 DNA. Western blot of podocin (upper band) confirms podocyte enrichment. (d) Coimmunostaining for nephrin (green), B7-1 (red, SC-9091 from Santa Cruz Biotechnology Inc.), and podocin (purple) in mouse podocytes 24 hours after exposure to either vehicle (upper panel) or 50.0 μg/ml LPS (lower panel). (e) B7-1 immunostaining using 3 anti-B7-1 antibodies (2A2: ab86473 from Abcam; AF140: from R&D Systems; and H-208: SC-9091 from Santa Cruz Biotechnology Inc.) in HeLa cells transfected with plasmids containing either an empty vector or mouse B7-1 DNA. Bar = 10 μm. The data are represented as the means ± SEM. Analysis of variance followed by a Tukey-Kramer test; podocytes treated with vehicle versus LPS: *** P < 0.001. DAPI, 4′,6-diamidino-2-phenylindole. Kidney International 2016 90, 1037-1044DOI: (10.1016/j.kint.2016.06.022) Copyright © 2016 International Society of Nephrology Terms and Conditions

Figure 2 B7-1 is not induced in immortalized podocytes after injury. (a) B7-1 mRNA is barely detectable in immortalized podocytes at baseline and is not induced after lipopolysaccharide (LPS) stimulation. IL-6 mRNA confirmed Toll-like receptor 4 engagement after LPS treatment. (b) Western blot of B7-1 (SC-9091 from Santa Cruz Biotechnology Inc.) in immortalized podocytes exposed to either vehicle or 50.0 μg/ml LPS for 24 hours. C+: positive control, HeLa cells transfected with a vector containing mouse B7-1 DNA. (c) Coimmunostaining for nestin (red), nephrin (green), and 3 different anti-B7-1 antibodies (purple, 2A2: ab86473 from Abcam [Paris, France]; AF140: from R&D Systems [Lille, France]; and H-208: SC-9091 from Santa Cruz Biotechnology Inc.) in immortalized podocytes 24 hours after exposure to either vehicle (upper panel) or 50.0 μg/ml LPS (lower panel). Bar = 10 μm. The data are represented as the means ± SEM. Analysis of variance followed by a Tukey-Kramer test; podocytes treated with vehicle versus LPS: *** P < 0.001. DAPI, 4′,6-diamidino-2-phenylindole. Kidney International 2016 90, 1037-1044DOI: (10.1016/j.kint.2016.06.022) Copyright © 2016 International Society of Nephrology Terms and Conditions

Figure 3 B7-1 is not induced in murine models of podocyte injury. (a) The albumin-creatinine ratio in the different models of podocytes injuries. (b) Glomerular morphology in sham-operated mice, in mice injected with lipopolysaccharide (LPS) (day 1 post injection), in mice injected with Adriamycin (day 7 post injection), in NZB/W F1 mice (age 46 weeks), and in mice 2 months after nephrectomy (Nx). (c) Transmission electron microscopy of the glomerular basement membrane in kidneys of vehicle- or LPS-treated mice 1 day after injection. Bar = 0.5 μm. (d) Coimmunostaining for nestin (green) and 3 different anti-B7-1 antibodies (red, 2A2: ab86473 from Abcam; AF140: from R&D Systems; and H-208: SC-9091 from Santa Cruz Biotechnology Inc.) in the paraffin-embedded kidneys of all models of podocyte injuries. (e) Coimmunostaining for nestin (green) and anti-B7-1 antibody (red, AF140: from R&D Systems) in the frozen sections of kidney of all models of podocyte injuries. Bar = 10 μm. Analysis of variance followed by a Tukey-Kramer test; all models versus sham: *** P < 0.001. DAPI, 4′,6-diamidino-2-phenylindole. Kidney International 2016 90, 1037-1044DOI: (10.1016/j.kint.2016.06.022) Copyright © 2016 International Society of Nephrology Terms and Conditions