Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

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Presentation transcript:

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska, MS, Xiaoping Zhu, MD, PhD, Anuk M. Das, PhD, Francis Poulain, MD, Angela Haczku, MD, PhD Journal of Allergy and Clinical Immunology Volume 122, Issue 3, Pages 521-528 (September 2008) DOI: 10.1016/j.jaci.2008.05.002 Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Expression of TNF-α in the airways was associated with increased numbers of macrophages, and it preceded enhanced production of SP-D. A, BALB/c mice were sensitized and challenged with Aspergillus fumigatus (Af) and studied before (0) and after challenge. B, Constitutive TNF-α expression was targeted into lung type II alveolar epithelial cells by using an SP-C promoter construct. C, Mice were injected with anti-TNF-α or isotype control antibodies (0.5 mg) immediately before allergen challenge and were studied 24 hours later. Total and differential cell counts were assessed, and TNF-α and SP-D protein release was analyzed by means of ELISA (Fig 1, A and B) and Western blotting (Fig 1, C; 3 representative bands of n = 6 in each group). TNF-α (Fig 1, B) and SP-D (Fig 1, C) mRNA expression in the lung tissue was studied by means of real-time PCR. Results are presented as means ± SEMs (n ≥ 6). ∗P < .05. N, Naive mice; Wt, wild-type; TNFα-tg, TNF-α–transgenic mice; MP, macrophage; EP, eosinophil; NP, neutrophil; LC, Lymphocyte. Journal of Allergy and Clinical Immunology 2008 122, 521-528DOI: (10.1016/j.jaci.2008.05.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 Enhanced SP-D expression in the airways is directly stimulated by IL-13. A, BALB/c mice were sensitized and challenged with Aspergillus fumigatus (Af) and studied at the indicated time points. B, Constitutive TNF-α expression was targeted into lung type II alveolar epithelial cells by using an SP-C promoter construct. C, Recombinant mouse IL-13 (1.5 μg) or BSA was administered intratracheally to sensitized mice, and BAL fluid SP-D levels were studied 24 hours later. D, Isolated rat type II alveolar epithelial cells were cultured with IL-13 for 4 days. E, IL-13–deficient mice were sensitized and challenged with the allergen and studied 24 hours later. IL-13 protein in BAL fluid (Fig 2, A) was assessed by means of ELISA. IL-13 mRNA (Fig 2, A and B) in the lung was studied by means of real-time PCR. SP-D protein content was analyzed by using Western blotting (Fig 2, C-E). Results are presented as means ± SEMs (n ≥ 6). ∗P < .05. N, Naive mice; Wt, wild-type, TNFα-tg, TNF-α–transgenic mice. Journal of Allergy and Clinical Immunology 2008 122, 521-528DOI: (10.1016/j.jaci.2008.05.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 3 Lack of SP-D in gene-deficient (SP-D−/−) mice resulted in abnormal count, morphology, and activation of CD11c-expressing cells in the BAL fluid. A, Total counts of BAL fluid cells from wild-type (Wt) and SP-D−/− mice (mean ± SEM of n = 8). B, Morphologic differences between Wild-type and SP-D−/− BAL fluid samples by means of FACS analysis (left panels) and light microscopic analysis of cytospin preparations (right panels). C and D, Expression of the dendritic cell and macrophage marker CD11c on wild-type and SP-D−/− BAL fluid cells, as assessed by labeling with phycoerythrin-conjugated anti-mouse mAb by means of FACS analysis (Fig 3, C) and immunocytochemistry (Fig 3, D). E, CD11c+ cells were gated in the BAL fluid of SP-D−/− and wild-type mice and analyzed for expression of MHC class II, CD86, and CD11b by using mAbs. Fig 3, B through E: Representative samples of 3 independent experiments. Isotype controls were also used for MHC class II, CD86, and CD11b antibodies (data not shown). Journal of Allergy and Clinical Immunology 2008 122, 521-528DOI: (10.1016/j.jaci.2008.05.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 4 Lack of SP-D enhanced and presence of SP-D suppressed TNF-α expression in CD11c+ cells. A, Representative FACS histograms of intracellular TNF-α expression in CD11c/MHC class II/CD86–positive BAL fluid cells. B, Quantitative analysis of TNF-α in wild-type (Wt) and SP-D−/− BAL fluid cells. C, Cell-free BAL supernatant was analyzed for TNF-α in naive wild-type and SP-D−/− mice (mean ± SEM of n = 6). ∗P < .05. D and E, Bone marrow–derived c-kit+ cells were cultured as described. Fig 4, D: Cells were analyzed for morphologic characteristics by using carboxyfluorescein succinimidyl ester labeling of cytospin preparations (top panels) and FACS on day 0 (left panels) and day 10 (right panels). MHC class II, CD86, CD11b, and TNF-α expression was assessed on CD11c-gated cells. Fig 4, E: Recombinant mouse SP-D was added on day 4 of the culture. CD11c+ cells were analyzed for MHC class II, CD86, CD11b, and TNF-α expression on day 10 (mean ± SEM of n = 6). ∗P < .05, ∗∗P < .01. Journal of Allergy and Clinical Immunology 2008 122, 521-528DOI: (10.1016/j.jaci.2008.05.002) Copyright © 2008 American Academy of Allergy, Asthma & Immunology Terms and Conditions