Accumulation of Identical T Cells in Melanoma and Vitiligo-Like Leukoderma Jürgen C. Becker, Per Guldberg, Jesper Zeuthen, Eva-Bettina Bröcker, Per thor.

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Accumulation of Identical T Cells in Melanoma and Vitiligo-Like Leukoderma Jürgen C. Becker, Per Guldberg, Jesper Zeuthen, Eva-Bettina Bröcker, Per thor Straten Journal of Investigative Dermatology Volume 113, Issue 6, Pages 1033-1038 (December 1999) DOI: 10.1046/j.1523-1747.1999.00805.x Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Immunohistologic detection of Vβ2 + T cells in melanoma and leukoderma. (A) Clinical presentation, scale bar: 10 mm, samples take for molecular analysis are indicated; (B) hematoxylin and eosin staining, scale bar: 1 mm; Immunohistology of (C, E, G) the tumor and (D, F, H) the halo stained with an (C, D) anti-MART-1/Melan-A, (E, F) anti-Vβ2-, or (G, H) anti-Vβ8-antibody. Scale bar: (A) 10 mm; (B) 250 μm; (C, E, F, G, H) 25 μm; (D) 100 μm. Journal of Investigative Dermatology 1999 113, 1033-1038DOI: (10.1046/j.1523-1747.1999.00805.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 RT-PCR/DGGE in situ TCR clonotype mapping covering BV families 1–24. cDNA from Melanoma lesion 2 was amplified with primers covering BV families 1–24. PCR products were loaded onto a 20% - 80% denaturing gradient gel and run for 4.5 h at 160 V at a constant temperature of 58°C. DNA was visualized by ethidium bromide staining and photographed under UV light. Journal of Investigative Dermatology 1999 113, 1033-1038DOI: (10.1046/j.1523-1747.1999.00805.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Presence of clonotypic TCR transcripts in melanoma and leukoderma. cDNA from PBL, tumor tissue, and the vitiligo-like leukoderma was amplified with primers specific for BV families 2, 8, 16, and 22. PCR products were subjected to a 20% - 80% denaturing gradient gel. DNA was visualized by ethidium bromide staining and photographed under UV light. Journal of Investigative Dermatology 1999 113, 1033-1038DOI: (10.1046/j.1523-1747.1999.00805.x) Copyright © 1999 The Society for Investigative Dermatology, Inc Terms and Conditions