Akt expression in mouse oocytes matured in vivo and in vitro

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Akt expression in mouse oocytes matured in vivo and in vitro Sandra Cecconi, Gianna Rossi, Adriana Santilli, Leonardo Di Stefano, Yumi Hoshino, Eimei Sato, Maria Grazia Palmerini, Guido Macchiarelli Reproductive BioMedicine Online Volume 20, Issue 1, Pages 35-41 (January 2010) DOI: 10.1016/j.rbmo.2009.10.011 Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Expression pattern of Akt isoforms in oocytes matured in vivo and in vitro in the presence of hypoxanthine-FSH or hypoxanthine-epidermal growth factor (EGF). (A) Germinal vesicle (GV) and metaphase I (MI), (B) metaphase II (MII). Representative pictures of reverse-transcriptase polymerase chain reaction experiments are shown. Statistical analysis was made on three different experiments. Data are represented as mean ± SEM of the relative ratio percentage between each Akt isoform and G3PDH. Significant differences (P < 0.05) are represented with different letters (a–c). bp, base pairs. Reproductive BioMedicine Online 2010 20, 35-41DOI: (10.1016/j.rbmo.2009.10.011) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Representative Western blot of Ser-473-phosphorylated Akt (pAkt) content in (A) metaphase I (MI) and (B) MII oocytes (100 oocytes/lane), matured in vivo and in vitro, either with hypoxanthine-FSH or hypoxanthine-epidermal growth factor (EGF). The ratio of pAkt to total Akt was determined for each condition. Results are expressed as the mean ± SEM of three separate experiments. Significant differences (P < 0.05) are represented with different letters (a–c). Reproductive BioMedicine Online 2010 20, 35-41DOI: (10.1016/j.rbmo.2009.10.011) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Localization of Ser-473-phosphorylated Akt and chromosomes in metaphase II oocytes matured (a) in vivo or (b and c) in vitro in the presence of hypoxanthine-FSH (b) and hypoxanthine-epidermal growth factor (c). 50–60 oocytes were analysed for each experimental condition. Gray and white represent phosphorylated Akt and chromosome, respectively. Arrows indicate the first polar body. Bar = 10 μm. Reproductive BioMedicine Online 2010 20, 35-41DOI: (10.1016/j.rbmo.2009.10.011) Copyright © 2009 Reproductive Healthcare Ltd. Terms and Conditions