Proton NMR Detection of Porphyrins and Cytochrome c in Small Unilamellar Vesicles: Role of the Dissociation Kinetic Constant Gregory Da Costa, Soizic.

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Proton NMR Detection of Porphyrins and Cytochrome c in Small Unilamellar Vesicles: Role of the Dissociation Kinetic Constant Gregory Da Costa, Soizic Chevance, Elisabeth Le Rumeur, Arnaud Bondon Biophysical Journal Volume 90, Issue 8, Pages L55-L57 (April 2006) DOI: 10.1529/biophysj.106.081521 Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 1 Low-field portion of 500-MHz 1H NMR spectra acquired at 313K at pD 7.5 with the PASE method, using 800μM of bis-cyano-hemin with increasing lipid molar ratio. Biophysical Journal 2006 90, L55-L57DOI: (10.1529/biophysj.106.081521) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 2 Portions of 1H NMR spectra at 323K corresponding to the meso-protons, using 700μM molar hematoporphyrin at various porphyrin: DOPC SUV ratios and different pD; intensities are arbitrary. (A) fully associated hematoporphyrin, molar ratio 1:100 and pD 7.5; (B) partially associated, molar ratio 1:25 at pD 7.5, and (C) at pD 10.5. Biophysical Journal 2006 90, L55-L57DOI: (10.1529/biophysj.106.081521) Copyright © 2006 The Biophysical Society Terms and Conditions

Figure 3 Low-field portion of 500MHz 1H NMR spectra of 500μM ferri-cyt c. (A) native; (B) cyano adduct; (C) cyano adduct with SDS micelles, 1:100; and (D) cyano adduct with DOPS/DOPC (2:1) SUV at a molar ratio of 1:150. Biophysical Journal 2006 90, L55-L57DOI: (10.1529/biophysj.106.081521) Copyright © 2006 The Biophysical Society Terms and Conditions