Volume 9, Issue 3, Pages (March 2002)

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Volume 9, Issue 3, Pages 367-373 (March 2002) Hexaene Derivatives of Nystatin Produced as a Result of an Induced Rearrangement within the nysC Polyketide Synthase Gene in S. noursei ATCC 11455 Trygve Brautaset, Per Bruheim, Håvard Sletta, Lars Hagen, Trond E Ellingsen, Arne R Strøm, Svein Valla, Sergey B Zotchev Chemistry & Biology Volume 9, Issue 3, Pages 367-373 (March 2002) DOI: 10.1016/S1074-5521(02)00108-4

Figure 1 Role of the NysC PKS in the Biosynthesis of the Nystatin Macrolactone Ring KS: β-ketoacyl synthase, AT: acyl transferase, DH: dehydratase, ER: enoyl reductase, KR: ketoreductase, ACP: acyl carrier protein. Chemistry & Biology 2002 9, 367-373DOI: (10.1016/S1074-5521(02)00108-4)

Figure 2 Analysis of Metabolites Produced by the S. noursei Wild-Type and ERD48 Strains (A) A UV scan of DMSO extracts of S. noursei ATCC 11455 and ERD48 strains. (B) Diode-array HPLC data for the ERD48 culture extract. Molecular weights (MW) for three major compounds determined by LC-MS/MS are indicated (see text for details). Chemistry & Biology 2002 9, 367-373DOI: (10.1016/S1074-5521(02)00108-4)

Figure 3 Preliminary Structural Characterization of the Hexaene Nystatin Derivative S48HX (A and B) Fragmentation patterns for the (A) S48HX compound and (B) nystatin obtained during LC-MS/MS analysis of the culture extracts from the ERD48 mutant and the WT strain, respectively. Fragments correlated by Δ28 and described in the Results are indicated with arrows. (C) Proposed planar chemical structure of the S48HX compound. Chemistry & Biology 2002 9, 367-373DOI: (10.1016/S1074-5521(02)00108-4)

Figure 4 Model Explaining the Generation of the ERD48 Mutant After integration of the pERD4.2 vector into the nysC gene, a second crossover between highly homologous KR4- and KR5-encoding sequences might take place. This event results in the elimination of one complete module from the NysC PKS (NysCΔ48). Chemistry & Biology 2002 9, 367-373DOI: (10.1016/S1074-5521(02)00108-4)