Duodenal expression of a putative stimulator of Fe transport and transferrin receptor in anemia and hemochromatosis  Donatella Barisani, Dario Conte  Gastroenterology  Volume 120, Issue 6, Pages 1404-1411 (May 2001) DOI: 10.1053/gast.2001.23946 Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 1 Western blot analysis using anti-SFT antibody in controls and subjects with HFE-related hemochromatosis or non–HFE-related iron overload. Fifty micrograms of proteins were separated on an 8% sodium dodecyl sulfate–polyacrylamide gel electrophoresis under reducing conditions and electroblotted onto ECL membranes. Primary and secondary antibodies were used at 1:500 and 1:10,000 dilution, respectively. (A) Controls; (B) HFE-related hemochromatosis; (C) non–HFE-related iron overload. Gastroenterology 2001 120, 1404-1411DOI: (10.1053/gast.2001.23946) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 2 Duodenal expression of the protein identified by the anti-SFT antibody in controls. (A) Protein localization in the middle and upper part of the villi. (B) The staining in the villus cells was mainly localized in the subapical part of the cytoplasm. (C) Lack of staining after hybridization on biopsy specimens from normal subjects with anti-SFT antibodies pretreated with SFT peptide. (Original magnifications: A, C, 40×; B, 100×.) Gastroenterology 2001 120, 1404-1411DOI: (10.1053/gast.2001.23946) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 3 Duodenal expression of the putative SFT in controls, patients with iron-deficiency anemia, and patients with iron overload. (A) In controls, staining was localized in the middle and upper part of the villi. (B) In patients with iron-deficiency anemia, staining was strongly increased throughout the entire villus. (C) In patients with HFE-related hemochromatosis, staining was slightly increased, extending at the lower third of the villus. (D) In patients with non–HFE-related iron overload, staining was detectable only at the top of the villi. (Original magnification 40× in all panels). Gastroenterology 2001 120, 1404-1411DOI: (10.1053/gast.2001.23946) Copyright © 2001 American Gastroenterological Association Terms and Conditions

Fig. 4 Duodenal expression of TfR in controls, patients with iron-deficiency anemia, and patients with iron overload. (A) In controls, TfR protein was mainly localized in the crypts, with faint staining in the villi. (B) In patients with iron-deficiency anemia, TfR expression was strongly increased in the crypts and throughout the entire villus. (C) In patients with HFE-related hemochromatosis, TfR expression was increased, extending from the crypts up to the top of the villus. (D) In patients with non–HFE-related iron overload, TfR was present mainly in the crypts. (Original magnification 40× in all panels.) Gastroenterology 2001 120, 1404-1411DOI: (10.1053/gast.2001.23946) Copyright © 2001 American Gastroenterological Association Terms and Conditions