DNA Extraction Experiment

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Presentation transcript:

DNA Extraction Experiment (One data sheet per group)

Introduction Today, you will extract DNA from a strawberry! Strawberries have 8 copies of each type of chromosome. This large number of chromosomes will filter out of your solution and you will get to actually see DNA.

Directions Place one strawberry in a ziploc bag (remove stem first!) Smash/grind the strawberry using your fist and fingers for 2 minutes. Careful not to break the bag!! Add 10mL of the extraction buffer (water/salt/soap solution)

Directions Cont’d Kneed/mush the strawberry in the bag for 1 more minute. Assemble your filtration apparatus Pour the strawberry slurry into the filtration apparatus and let it drip directly into your test tube.

Slowly pour 20mL of cold ethanol into the test tube Dip the straw into the tube where the strawberry extract and ethanol layers come into contact with each other. Stir it around gently.

Conclusions: Complete the questions on the back of the data sheet!

Strawberry DNA