Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass.

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Presentation transcript:

Rapid method for direct identification of bacteria in urine and blood culture samples by matrix-assisted laser desorption ionization time-of-flight mass spectrometry: intact cell vs. extraction method L. Ferreira, F. Sánchez-Juanes, J.L. Muñoz-Bellido, J.M. González-Buitrago Clinical Microbiology and Infection Volume 17, Issue 7, Pages 1007-1012 (July 2011) DOI: 10.1111/j.1469-0691.2010.03339.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Diagram of the general workflow in the analysis of urine and blood culture samples by matrix-assisted laser desorption ionization (MALDI) time-of-flight mass spectrometry. Clinical Microbiology and Infection 2011 17, 1007-1012DOI: (10.1111/j.1469-0691.2010.03339.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2. Comparison of the intact cell method and the extraction method in urine and blood culture samples analysed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The samples compared are those with coincidence at the genus level with plate culture and biochemical identification. Clinical Microbiology and Infection 2011 17, 1007-1012DOI: (10.1111/j.1469-0691.2010.03339.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3. Mass spectra (size 2.000–11.000 Da) of Proteus mirabilis and Klebsiella pneumoniae from urine samples obtained with the intact cell method (a) and with the protein extraction method (b). a.u., arbitrary units. Clinical Microbiology and Infection 2011 17, 1007-1012DOI: (10.1111/j.1469-0691.2010.03339.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions