Y.R. Fu, Z.J. Yi, S.Z. Guan, S.Y. Zhang, M. Li

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Presentation transcript:

Proteomic analysis of sputum in patients with active pulmonary tuberculosis Y.R. Fu, Z.J. Yi, S.Z. Guan, S.Y. Zhang, M. Li Clinical Microbiology and Infection Volume 18, Issue 12, Pages 1241-1247 (December 2012) DOI: 10.1111/j.1469-0691.2012.03824.x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1 Protein patterns of sputum. (a) A representative TB example is shown. (b) A representative control sample is shown. The resulting two samples containing 150 μg protein were added to pH 3–10, non-linear DryStrips (24 cm long; Bio-Rad, Hercules, CA, USA). The p values for these spots in three independent experiments were <0.05. Clinical Microbiology and Infection 2012 18, 1241-1247DOI: (10.1111/j.1469-0691.2012.03824.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 2 The result of the matrix-assisted laser desorption/ionization-time of flight/time of flight-mass spectrometry MALDI-TOF/TOF-MS analysis of the protein IL-25. It was identified to be human IL-25 (NCBI accession number: 18034676) by protein database search. (a) Peptide mass fingerprint. (b) MS/MS profile of the peptide with a mass of 1045.56 Da; amino acids indicated as ASEDGPLNSR; confidence score 99.783%. Clinical Microbiology and Infection 2012 18, 1241-1247DOI: (10.1111/j.1469-0691.2012.03824.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 3 Confirmation of IL-25 expression by ELISA. The data showed that IL-25 had an increased abundance in the TB group compared with the control group. #The p value for IL-25 was <0.05 in the active TB group compared with the controls. Clinical Microbiology and Infection 2012 18, 1241-1247DOI: (10.1111/j.1469-0691.2012.03824.x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions